Anti-herpes virus activities of Achyranthes aspera: An Indian ethnomedicine, and its triterpene acid
Anti-herpes virus activities of Achyranthes aspera: An Indian ethnomedicine, and its triterpene acid
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DOI：
10.1016/j.micres.2012.11.002
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发表时间：
2013-01-01
影响因子：
6.7
通讯作者：
Chattopadhyay, Debprasad
中科院分区：
文献类型：
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作者：
Mukherjee, Hemanta;Ojha, Durbadal;Chattopadhyay, Debprasad
本研究的目的是评价印度民间药牛膝醇提物(ME)及其纯化合物齐墩果酸(OA)对单纯疱疹病毒1型(HSV-1)和2型(HSV-2)的抗病毒作用。ME具有较弱的抗疱疹病毒活性(EC50对HSV-1为64.4 mU/ml，对HSV-2为72.8 mU/ml)。而OA对HSV-1(EC50为6.8 mU/ml)和HSV-2(EC50为7.8 mU/ml)均有较强的抗疱疹病毒活性。时间效应研究表明，ME和OA在感染后2-6h的抗病毒活性最强。感染和药物处理的小鼠腹腔巨噬细胞在特定时间段的促炎细胞因子(IL-6和IL-12)水平升高。此外，在不同的时间间隔，用ME和OA处理的感染细胞的DNA在48-72h都没有扩增，与用阿昔洛韦处理的HSV感染的细胞相似，这表明ME和OA可能抑制了早期(感染后2-6h)的增殖。因此，我们的研究表明，ME和OA具有良好的抗HSV活性，其SI值为12，表明该植物具有潜在的用途。(C)爱思唯尔股份有限公司出版的2012年。
The aim of this study was to evaluate the antiviral potential of methanolic extract (ME) of Achyranthes aspera, an Indian folk medicine and one of its pure compound oleanolic acid (OA) against herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2). The ME possessed weak anti-herpes virus activity (EC50 64.4 mu g/ml for HSV-1 and 72.8 mu g/ml for HSV-2). While OA exhibited potent antiherpesvirus activity against both HSV-1 (EC50 6.8 mu g/ml) and HSV-2 (EC50 7.8 mu g/ml). The time response study revealed that the antiviral activity of ME and OA is highest at 2-6 h post infection. The infected and drug-treated peritoneal macrophage at specific time showed increased level of pro-inflammatory cytokines (IL6 and IL12). Further, the PCR of DNA from infected cultures treated with ME and OA, at various time intervals, failed to show amplification at 48-72 h, similar to that of HSV infected cells treated with acyclovir, indicating that the ME and OA probably inhibit the early stage of multiplication (post infection of 2-6 h). Thus, our study demonstrated that ME and OA have good anti-HSV activity, with SI values of 12, suggesting the potential use of this plant. (C) 2012 Published by Elsevier GmbH.