Development of Arabidopsis thaliana transformants showing the self-recognition activity of Brassica rapa
Development of Arabidopsis thaliana transformants showing the self-recognition activity of Brassica rapa
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DOI：
10.1101/2020.07.15.205708
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发表时间：
2020-07
期刊：
影响因子：
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通讯作者：
Masaya Yamamoto;H. Kitashiba;T. Nishio
中科院分区：
文献类型：
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作者：
Masaya Yamamoto;H. Kitashiba;T. Nishio
菊科植物的自交不亲和性是由位于S位点的两个连锁的高度多态性基因SRK和SCR控制的。先前，将琴状拟南芥的SRK和SCR基因导入拟南芥转化体中以产生自交不亲和系。然而，目前还没有报道芸苔属植物的SRK和SCR基因在拟南芥中引起自交不亲和。thaliana.在本研究中，我们试图构建自交不亲和的A。通过引入BrSCR基因沿着嵌合BrSRK基因（BrSRK嵌合体，其中BrSRK的激酶结构域被AlSKRb的激酶结构域取代）表达芜菁自我识别活性的拟南芥转化体。我们发现B的BrSRK嵌合体和BrSCR。rapa S-9和S-46单倍型，而不是S-29，S-44和S-60单倍型，赋予了A. thaliana.我们还使用A.表达BrSRK-9嵌合体和BrSCR-9的突变体变体的拟南芥转化体。结果表明，一些氨基酸残基是自我识别所必需的。本研究建立的构建自交不亲和A。显示B的拟南芥转化体。rapa的自我识别活性将有助于分析菊科植物的自我识别机制。
Self-incompatibility in the Brassicaceae family is governed by two-linked highly polymorphic genes located at the S locus, SRK and SCR. Previously, the SRK and SCR genes of Arabidopsis lyrata were introduced into Arabidopsis thaliana transformants to generate self-incompatible lines. However, it has not been reported that the SRK and SCR genes of Brassica species confer self-incompatibility in A. thaliana. In this study, we attempted to construct self-incompatible A. thaliana transformants expressing the self-recognition activity of Brassica rapa by introducing the BrSCR gene along with a chimeric BrSRK gene (BrSRK chimera, in which the kinase domain of BrSRK was replaced with that of AlSKRb). We found that BrSRK chimera and BrSCR of B. rapa S-9 and S-46 haplotypes, but not those of S-29, S-44, and S-60 haplotypes, conferred self-recognition activity in A. thaliana. We also investigated the importance of amino acid residues involved in the BrSRK9–BrSCR9 interaction using A. thaliana transformants expressing mutant variants of BrSRK-9 chimera and BrSCR-9. The results showed that some of the amino acid residues are essential for self-recognition. The method developed in this study for the construction of self-incompatible A. thaliana transformants showing B. rapa self-recognition activity will be useful for analysis of self-recognition mechanisms in Brassicaceae.