Optical micro resonance based sensor schemes for detection and identification of nano particles and biological agents in situ
Optical micro resonance based sensor schemes for detection and identification of nano particles and biological agents in situ
复制标题
基于光学微共振的传感器方案，用于原位检测和识别纳米颗粒和生物制剂
DOI：
10.1117/12.853691
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发表时间：
2010
影响因子：
0.7
通讯作者：
A. Ostendorf
中科院分区：
文献类型：
--
作者：
V. Saetchnikov;E. Tcherniavskaia;G. Schweiger;A. Ostendorf
利用光学微腔共振技术对纳米粒子（包括生物分子）进行无标记分析是一种新兴的技术。基于微球的机械固定以及微球的激光熔化的标准单模光纤的尖端上的各种方案进行了研究，使微生物应用的进一步发展。乙醇，盐酸，葡萄糖，维生素C和生物素的水溶液已被用来测试折射率的变化，通过监测回音壁模式光谱位移的幅度。特别的努力是为了有效地固定在水流中的微球，微共振观察的最佳几何形状和微球的材料最适合微生物应用。在小于1微瓦的激光功率下，观察到由光刻法制作的固定在微通道中的PMMA自由微球中的光共振。本文研究了C反应蛋白水溶液和白蛋白水溶液以及盐酸模拟血液的共振位移。在传感器微球周围引入一定量的玻璃凝胶纳米粒子，伴随着相关共振位移（直径为400 nm）和共振谱的完全重建（直径为57 nm）。开发的方案已被证明是一个有前途的技术平台，用于不同生物分子的诊断工具的敏感的芯片上实验室型传感器，例如蛋白质、寡核苷酸、寡糖、脂质、小分子、病毒颗粒、细胞以及不同的实验环境，例如蛋白质组学、基因组学、药物发现和膜研究。
A novel emerging technique for the label-free analysis of nano particles including biomolecules using optical micro cavity resonance is being developed. Various schemes based on a mechanically fixed microspheres as well as microspheres melted by laser on the tip of a standard single mode fiber have been investigated to make further development for microbial application. Water solutions of ethanol, HCl, glucose, vitamin C and biotin have been used to test refractive index changes by monitoring the magnitude of the whispering gallery modes spectral shift. Particular efforts were made for effective fixing of the micro spheres in the water flow, an optimal geometry for micro resonance observation and material of microsphere the most appropriate for microbial application. Optical resonance in free micro spheres from PMMA fixed in micro channels produced by photolithography has been observed under the laser power of less then 1 microwatt. Resonance shifts of C reactive protein water solutions as well as albumin solutions in pure water and with HCl modelling blood have been investigated. Introducing controlled amount of glass gel nano particles into sensor microsphere surrounding were accompanied by both correlative resonance shift (400 nm in diameter) and total reconstruct of resonance spectra (57 nm in diameter). Developed schemes have been demonstrated to be a promising technology platform for sensitive, lab-on-chip type sensor of diagnostic tools for different biological molecules, e.g. proteins, oligonucleotides, oligosaccharides, lipids, small molecules, viral particles, cells as well as in different experimental contexts e.g. proteomics, genomics, drug discovery, and membrane studies.
影响因子：
3.8
作者：
White, Ian M.;Fan, Xudong
通讯作者：
Fan, Xudong
影响因子：
4
作者：
Pang, Shuo;Beckham, Richard E.;Meissner, Kenith E.
通讯作者：
Meissner, Kenith E.