Association between KIAA1199 overexpression and tumor invasion, TNM stage, and poor prognosis in colorectal cancer.
Association between KIAA1199 overexpression and tumor invasion, TNM stage, and poor prognosis in colorectal cancer.
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发表时间：
2015-03
影响因子：
1.4
通讯作者：
Jian Xu;Y. Liu;Xudong Wang;Jianfei Huang;Huijun Zhu;Zhiqian Hu;Defeng Wang
中科院分区：
文献类型：
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作者：
Jian Xu;Y. Liu;Xudong Wang;Jianfei Huang;Huijun Zhu;Zhiqian Hu;Defeng Wang
研究肿瘤组织中 KIAA1199 的表达及其作为结直肠癌 (CRC) 患者生存预后指标的潜在价值。使用实时 PCR 和 20 对新鲜冷冻的 CRC 组织和相应的非癌组织来表征 CRC 中 KIAA1199 mRNA 的表达。使用免疫组织化学在 202 名 CRC 患者的组织微阵列芯片上证实了 KIAA1199 蛋白的表达。然后，我们将 KIAA1199 蛋白表达与 CRC 常规临床病理特征和患者结果相关联。与正常组织相比，CRC中KIAA1199 mRNA和蛋白的表达上调（分别P=0.015和P<0.001）。 KIAA1199蛋白表达与肿瘤浸润深度（P=0.013）和淋巴结转移（P=0.003）相关。 Kaplan-Meier生存和Cox回归分析显示，KIAA1199高表达（P<0.001）和术后血清癌胚抗原（CEA）水平（P=0.005）是预测CRC患者预后不良的独立因素。我们提供的证据表明，KIAA1199 的高表达与 CRC 的肿瘤浸润深度、TNM 分期和不良预后相关。我们的研究结果表明 KIAA1199 可用作 CRC 的预后因素和新的治疗靶点。
To investigate the expression of KIAA1199 in tumor tissue and its potential value as a prognostic indicator of survival in patients with colorectal cancer (CRC). The expression of KIAA1199 mRNA in CRC was characterized using real-time PCR and 20 pairs of fresh-frozen CRC tissues and corresponding non-cancerous tissues. KIAA1199 protein expression was confirmed using immunohistochemistry on a tissue microarray chip from 202 patients with CRC. Then, we correlated KIAA1199 protein expression to CRC conventional clinicopathological features and patient's outcome. The expression of KIAA1199 mRNA and protein were up-regulated in CRC compared to normal tissues (P=0.015 and P<0.001, individually). KIAA1199 protein expression was related to tumor invasion depth (P=0.013) and lymph node metastasis (P=0.003). Kaplan-Meier survival and Cox regression analyses revealed that high KIAA1199 expression (P<0.001) and serum carcinoembryonic antigen (CEA) level post operation (P=0.005) were independent factors predicting poor prognosis of patients with CRC. We present evidence that high expression of KIAA1199 is associated with tumor invasion depth, TNM stage, and poor prognosis in CRC. Our findings suggest KIAA1199 could be used as a prognostic factor and novel therapeutic target for CRC.