CYTOPHOTOMETRIC REINVESTIGATION OF DNA CONTENT IN PURKINJE-CELLS OF THE RAT CEREBELLUM

CYTOPHOTOMETRIC REINVESTIGATION OF DNA CONTENT IN PURKINJE-CELLS OF THE RAT CEREBELLUM
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DOI:
10.1007/bf00533133
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发表时间:
1980-01-01
期刊:
HISTOCHEMISTRY
影响因子:
--
通讯作者:
VANDERPLOEG, M
VANDERPLOEG, M
中科院分区:
其他
文献类型:
--
作者:
MARES, V;VANDERPLOEG, M

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采用分期扫描细胞光度法对年轻成年大鼠小脑feulgen染色细胞核进行了研究。大多数浦肯野细胞的吸光度值呈高斯分布,方差系数较低。该群体的吸光度峰值与颗粒细胞相同。大约1%的浦肯野细胞的染色含量表明DNA含量为4C。对于颗粒状和浦肯野细胞群,极少数细胞核具有介于2C-4C之间的吸光度值。普遍的浦肯野细胞二倍体是支持的。不建议该种群为四倍体和/或超二倍体。
Feulgen-stained cell nuclei of the cerebellum of young adult rats were studied by stage scanning cytophotometry. The absorbance values of the majority of the Purkinje cells showed a Gaussian distribution with a low coefficient of variance. The peak absorbance of this population was the same as the granule cells. About 1% of the Purkinje cells measured had a stain content indicating a 4C amount of DNA. For the granular and Purkinje cell population a very small number of nuclei possesses absorbance values intermediate from 2C-4C. Prevalent diploidy of the Purkinje cells is supported. A tetraploid and/or hyperdiploid status of this population is not suggested.