Lipocortin 1 (annexin 1) in patches associated with the membrane of a lung adenocarcinoma cell line and in the cell cytoplasm.
Lipocortin 1 (annexin 1) in patches associated with the membrane of a lung adenocarcinoma cell line and in the cell cytoplasm.
复制标题
与肺腺癌细胞系膜相关的斑块和细胞质中的脂皮质素 1（膜联蛋白 1）。
作者：
V. Traverso;J. Morris;R. Flower;J. Buckingham
脂皮质蛋白1 （annexin I）是一种钙和磷脂结合的膜联蛋白，尽管缺乏信号序列，但可以从细胞外化。为了确定其在A549人肺腺癌细胞中的细胞分布，采用光镜和电镜免疫细胞化学、细胞分离和免疫印迹法对其进行了定位。脂皮质蛋白1的免疫反应性集中在与质膜相关的突出斑块上。这些斑块的强度随培养的融合和持续时间而变化，并且在固定前EDTA洗涤不会明显减弱。微管蛋白和细胞角蛋白8与脂皮质蛋白1在斑块中共定位。细胞内脂皮质素1分布在细胞质中。电镜显示沿质膜有明显的免疫反应性，偶有大簇金颗粒与细胞膜表面接触；在细胞质内，一些囊泡/液泡结构的膜和一些小的电子致密体具有免疫反应性，但没有免疫金颗粒与多层体相关。亚细胞分离、提取和western blotting结果表明，脂皮质蛋白1在膜颗粒中以两个不同的组分存在；一个与脂质双分子层密切相关，表现为整体膜蛋白，另一个松散附着，表现为外周膜蛋白。结果表明，大量的脂皮质蛋白1集中在与质膜相关的和紧挨着质膜的局灶结构中。这些可能构成了脂皮质素1从细胞中释放的部分机制。
Lipocortin 1 (annexin I) is a calcium- and phospholipid-binding annexin protein which can be externalised from cells despite the lack of a signal sequence. To determine its cellular distribution lipocortin 1 in A549 human lung adenocarcinoma cells was localised by light- and electron-microscopic immunocytochemistry and by cell fractionation and western blotting. Lipocortin 1 immunoreactivity is concentrated in prominent patches associated with the plasma membrane. The intensity of these patches varied with the confluence and duration of the culture and was not detectably diminished by an EDTA wash before fixation. Tubulin and cytokeratin 8 were colocalized with lipocortin 1 in the patches. Within the cells lipocortin 1 was distributed throughout the cytoplasm. Electron microscopy revealed prominent immunoreactivity along the plasma membrane with occasional large clusters of gold particles in contact with the membrane surface of the cells; within the cytoplasm the membrane of some vesicle/vacuole structures and some small electron-dense bodies was immunoreactive, but no immunogold particles were associated with the multilamellar bodies. Subcellular fractionation, extraction and western blotting showed that lipocortin 1 in the membrane pellet was present as two distinct fractions; one, intimately associated with the lipid bilayer, which behaved like an integral membrane protein and one loosely attached which behaved like a peripheral membrane protein. The results show that a substantial amounts of lipocortin 1 is concentrated in focal structures associated with and immediately beneath the plasma membrane. These might form part of the mechanism by which lipocortin 1 is released from the cells.
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DOI：
10.1152/ajplung.1996.270.4.l668
发表时间：
1996-04-01
影响因子：
4.9
作者：
Liu, L;Wang, MS;Zimmerman, UJP
通讯作者：
Zimmerman, UJP
兔肺钙依赖性磷脂结合蛋白的免疫表征和发育调节。
DOI：
10.1016/0005-2760(94)90226-7
发表时间：
1994
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Tsao,FH;Chen,X;Chen,X;Vu,VX
通讯作者：
Vu,VX
大鼠肺泡上皮 II 型细胞中蛋白水解对膜联蛋白 I 的调节。
DOI：
--
发表时间：
1995
期刊：
Biochemistry and molecular biology international.
影响因子：
--
作者：
Liu,L;Fisher,AB;Zimmerman,UJ
通讯作者：
Zimmerman,UJ
影响因子：
2.9
作者：
L. Varticovski;S. Chahwala;M. Whitman;L. Cantley;Daniel Schindler-;E. Chow;L. K. Sinclair;R. Pepinsky
通讯作者：
L. Varticovski;S. Chahwala;M. Whitman;L. Cantley;Daniel Schindler-;E. Chow;L. K. Sinclair;R. Pepinsky
影响因子：
4.1
作者：
Goulding, NJ;Pan, LY;Guyre, PM
通讯作者：
Guyre, PM