Brachyury is expressed in gastrulating bovine embryos well ahead of implantation
Brachyury is expressed in gastrulating bovine embryos well ahead of implantation
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DOI：
10.1007/s004270100138
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发表时间：
2001-03-01
影响因子：
2.4
通讯作者：
Viebahn, C
中科院分区：
文献类型：
--
作者：
Hue, I;Renard, JP;Viebahn, C
Brachyury是一种含有T盒的转录因子，在脊椎动物原肠胚形成过程中参与中胚层的形成。为了分析原肠胚形成的调节是否与原肠胚形成的时间一样多地与植入有关，我们分离了牛brachyury cDNA片段。氨基酸序列显示与小鼠和人Brachyury的高度相似性和与醚T-box基因的明显差异。整体安装原位杂交揭示了一个正常的表达模式，除了一个短暂的减少表达的原始条纹的前部。根据这些结果，哺乳动物的原肠胚形成与着床无关。
Brachyury is a T-box-containing transcription factor involved in mesoderm formation during vertebrate gastrulation. To analyse whether the regulation of gastrulation varies as much as the timing of gastrulation does with respect to implantation, we isolated a bovine brachyury cDNA fragment. The amino acid sequence shows high similarity to mouse and human Brachyury and clear differences to ether T-box genes. Whole-mount in situ hybridisation reveals a normal expression pattern except for a transiently reduced expression in the anterior part of the primitive streak. According to these results, gastrulation in mammals is implemented and regulated irrespective of implantation.