Attachment of Flagellar Basal Bodies to the Cell Envelope: Specific Attachment to the Outer, Lipopolysaccharide Membrane and the Cytoplasmic Membrane
Attachment of Flagellar Basal Bodies to the Cell Envelope: Specific Attachment to the Outer, Lipopolysaccharide Membrane and the Cytoplasmic Membrane
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鞭毛基体与细胞膜的附着：与外膜、脂多糖膜和细胞质膜的特异性附着
作者：
M. DePamphilis;J. Adler
描述了一种用于纯化仍附着有鞭毛的大肠杆菌外膜（脂多糖或L膜）的方法。所得脂多糖膜呈囊泡形式，其在薄切片中具有三层结构，并且含有约55%的脂多糖和45%的蛋白质。T_2或T_4噬菌体预吸附E.发现大肠杆菌附着在纯化的脂多糖膜上。鞭毛与纯化的脂多糖膜特异性地结合在最靠近钩的基体环（L环）处。在E.大肠杆菌原生质球或枯草芽孢杆菌原生质体特异性地附着在鞭毛上离钩最远的基体环（M环）上。在急诊大肠杆菌制备，脂多糖膜也存在，并连接到L环。从这些数据和E的结构和尺寸的知识。coli鞭毛基体和细胞被膜，推导出鞭毛附着模型。
A procedure is described for the purification of the Escherichia coli outer membrane (lipopolysaccharide or L membrane) with flagella still attached. The resulting lipopolysaccharide membrane was in the form of vesicles that had a trilaminar structure in thin section and contained about 55% lipopolysaccharide and 45% protein. T2 or T4 phage preadsorbed to E. coli were found attached to the purified lipopolysaccharide membrane. Flagella were bound to the purified lipopolysaccharide membrane specifically at the basal body ring closest to the hook (the L ring). The cytoplasmic membrane in preparations from osmotically lysed E. coli spheroplasts or Bacillus subtilis protoplasts was specifically attached to flagella at the basal body ring farthest from the hook (the M ring). In the E. coli preparation, lipopolysaccharide membrane was also present and was attached to the L ring. From these data and a knowledge of the structure and dimensions of the E. coli flagellar basal body and cell envelope, a model for flagellar attachment is deduced.