Increased expression of estrogen receptor-β mRNA in male blood vessels after vascular injury
Increased expression of estrogen receptor-β mRNA in male blood vessels after vascular injury
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DOI：
10.1161/01.res.83.2.224
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发表时间：
1998-07-27
影响因子：
20.1
通讯作者：
Mendelsohn, ME
中科院分区：
文献类型：
--
作者：
Lindner, V;Kim, SK;Mendelsohn, ME
雌激素对血管内皮细胞和平滑肌细胞发挥直接作用，这对血管保护很重要。雌激素受体-α（ER α）在男性和女性的血管细胞中表达，并可能介导雌激素对血管组织的某些作用。然而，我们最近发现雌激素能够保护卵巢切除雌性ER α基因敲除小鼠免受血管损伤。这些小鼠在其睾丸中表达新描述的雌激素受体-β（ER β），这表明ER β也可能介导雌激素对血管系统的一些直接作用。在这项研究中，使用en face（Hautchen）制备物和原位杂交检查了雄性大鼠睾丸中ER α和ER β mRNA的表达水平。在任何时间点，在血管内皮细胞或平滑肌细胞中观察到血管损伤后ER α表达几乎没有变化或没有变化。而ER β mRNA在球囊损伤后表达明显。在内皮细胞中，ER β在损伤后2天增加，并且在8天和14天维持高水平的表达。此外，ER β在损伤后8天和14天在管腔平滑肌细胞中表达较高，并在损伤后28天降至低水平。这些数据证明了ER β在男性血管组织中的存在和血管损伤后ER β mRNA表达的诱导，支持ER β在雌激素的直接血管效应中的作用。
Estrogen exerts direct effects on vascular endothelial and smooth muscle cells that are important for vascular protection. Estrogen receptor-alpha (ER alpha) is expressed in vascular cells from males and females and may mediate some of the effects of estrogen on vascular tissue. However, we recently found that estrogen is able to protect against vascular injury in ovariectomized female ER alpha knockout mice. These mice express the newly described estrogen receptor-beta (ER beta) in their aortas, suggesting that ER beta may also mediate some of the direct effects of estrogen on the vasculature, In this study, the level of expression of ER alpha and ER beta mRNA in male rat aortas was examined before and after vascular injury using en face (Hautchen) preparations and in situ hybridization. Little or no change in ER alpha expression was observed after vascular injury in either vascular endothelial or smooth muscle cells at any time point. In contrast, ER beta mRNA was found to be expressed markedly after balloon injury. In endothelial cells, ER beta was increased by 2 days after injury, and high levels of expression were maintained at 8 and 14 days. Furthermore, ER beta expression was high in luminal smooth muscle cells at 8 and 14 days after injury and had decreased to low levels by 28 days after injury. These data demonstrate the presence of ER beta in male vascular tissues and the induction of ER beta mRNA expression after vascular injury, supporting a role for ER beta in the direct vascular effects of estrogen.