Genetic linkage map of the interspecific grape rootstock cross Ramsey (Vitis champinii) x Riparia Gloire (Vitis riparia)
Genetic linkage map of the interspecific grape rootstock cross Ramsey (Vitis champinii) x Riparia Gloire (Vitis riparia)
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DOI：
10.1007/s00122-006-0264-8
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发表时间：
2006-05-01
影响因子：
5.4
通讯作者：
Walker, M. A.
中科院分区：
文献类型：
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作者：
Lowe, K. M.;Walker, M. A.
利用Ramsey （Vitis champinii）与Riparia Gloire （V. Riparia）杂交的188个后代构建了首个砧木葡萄亲本遗传连锁图谱。在354个简单序列重复标记中，205个标记对至少一个亲本具有多态性，57.6%的标记具有完全信息性。Ramsey， Riparia Gloire和F1种群的地图是使用JoinMap软件创建的，遵循伪测试交叉策略。205个ssr位点可鉴定全部19个Vitis连锁组（2n=38），总组合图谱长度为1,304.7 cM，标记间平均长度为6.8 cM。母体图谱由172个标记组成，排列成19个连锁组（1,244.9 cM），而父系图谱上的126个标记覆盖18个连锁组（1,095.5 cM）。预期的基因组覆盖率超过92%。在Ramsey、Riparia Gloire和consensus map中，分别有10%、13%和16%的标记发生了分离扭曲。这些扭曲的标记主要聚集在连锁群3、5、14和17上。基于315个共同标记间隔，Ramsey和Riparia Gloire的全基因组重组率没有差异。54个新的vitis - est衍生SSR标记在19个连锁群中的16个上均匀分布。这两种表型不同的北美葡萄品种的密集连锁图谱为研究许多砧木性状的遗传学提供了有价值的工具，包括抗线虫性、耐石灰和耐盐性以及诱导活力的能力。
The first genetic linkage map of grape derived from rootstock parents was constructed using 188 progeny from a cross of Ramsey (Vitis champinii) x Riparia Gloire (V. riparia). Of 354 simple sequence repeat markers tested, 205 were polymorphic for at least one parent, and 57.6% were fully informative. Maps of Ramsey, Riparia Gloire, and the F1 population were created using JoinMap software, following a pseudotestcross strategy. The set of 205 SSRs allowed for the identification of all 19 Vitis linkage groups (2n=38), with a total combined map length of 1,304.7 cM, averaging 6.8 cM between markers. The maternal map consists of 172 markers aligned into 19 linkage groups (1,244.9 cM) while 126 markers on the paternal map cover 18 linkage groups (1,095.5 cM). The expected genome coverage is over 92%. Segregation distortion occurred in the Ramsey, Riparia Gloire, and consensus maps for 10, 13, and 16% of the markers, respectively. These distorted markers clustered primarily on the linkage groups 3, 5, 14 and 17. No genome-wide difference in recombination rate was observed between Ramsey and Riparia Gloire based on 315 common marker intervals. Fifty-four new Vitis-EST-derived SSR markers were mapped, and were distributed evenly across the genome on 16 of the 19 linkage groups. These dense linkage maps of two phenotypically diverse North American Vitis species are valuable tools for studying the genetics of many rootstock traits including nematode resistance, lime and salt tolerance, and ability to induce vigor.