MORPHOLOGICAL RESPONSE OF WITCHWEED (STRIGA-ASIATICA) TO IN-VITRO CULTURE
MORPHOLOGICAL RESPONSE OF WITCHWEED (STRIGA-ASIATICA) TO IN-VITRO CULTURE
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DOI：
10.1093/jxb/44.8.1377
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发表时间：
1993-08-01
影响因子：
6.9
通讯作者：
BUTLER, LG
中科院分区：
文献类型：
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作者：
CAI, T;BABIKER, AG;BUTLER, LG
将切除的高粱根段（长度5-10 mm）在含有矿物盐、维生素、氨基酸、葡萄糖和IAA的四种不同液体培养基中培养50天。除去根，剩余的培养基用等体积的温热的1.6%水琼脂固化。将干燥的未调理或调理的独脚金种子转移到培养基中。有些种子发芽并发育成寄生型幼苗。这些幼苗具有吸器、小瘤、浓密根毛、分枝枝和多个不定根。胚芽极发育成一个芽，但胚根极显示只有初步的发展。在相同的培养基上，但以前没有用于生长高粱根，幼苗显示出发达的胚根衍生的根系，但胚芽不生长。培养35-50 d后，根上开始出现新梢。这些幼苗没有吸器，没有结节，根毛很少或分散，没有茎生不定根，茎枝很少，似乎是非寄生型幼苗。芽在附加IAA和激动素的培养基上生长，而在附加NAA和IBA的培养基上不生长。蔗糖和2，4-D分别替代葡萄糖和IAA，独脚金种子萌发，心形胚脱分化成愈伤组织。愈伤组织经继代培养9个月以上，结果表明，寄主信号，除了那些发芽和吸器形成，需要进一步发展。此外，培养的S.积雪草受到外源生长调节剂的影响。
Excised sorghum root segments (5-10 mm in length) were cultured for 50 d in four different liquid media containing mineral salts, vitamins, amino acids, glucose, and IAA. The roots were removed and the remaining medium was solidified with an equal volume of warm 1.6% water agar. Dry unconditioned or conditioned Striga asiatica seeds were transferred to the medium. Some of the seeds germinated and developed into parasitic-type seedlings. These seedlings had haustoria, tubercles, dense root hairs, branched shoots, and multiple shoot-borne adventitious roots. The plumule pole developed into a shoot, but the radicle pole displayed only rudimentary development. On the same media, but which had not previously been used to grow sorghum roots, the seedlings displayed a well-developed radicle-derived root system, but the plumule did not grow. Shoots began to appear on the roots only after 35-50 d of culture. These seedlings had no haustoria, no tubercles, few or scattered root hairs, no shoot-borne adventitious roots and few shoot branches, and appeared to be non-parasitic-type seedlings. Shoots grew in a medium supplemented with IAA and kinetin, but did not in a medium containing NAA plus IBA. On replacement of glucose and IAA with sucrose and 2,4-D, respectively, Striga seeds germinated, and the heart-shaped embryos dedifferentiated into calli. The calli have been maintained by subculturing for over 9 months. The results demonstrated that a host signal, in addition to those for germination and haustorium formation, is required for further development. Moreover, morphogenesis of cultured S. asiatica is influenced by exogenous growth regulators.