Advantage of gene gun-mediated over intramuscular inoculation of plasmid DNA vaccine in reproducible induction of specific immune responses
Advantage of gene gun-mediated over intramuscular inoculation of plasmid DNA vaccine in reproducible induction of specific immune responses
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DOI：
10.1016/s0264-410x(99)00432-6
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发表时间：
2000-03-06
期刊：
影响因子：
5.5
通讯作者：
Koide, Y
中科院分区：
文献类型：
--
作者：
Yoshida, A;Nagata, T;Koide, Y
利用编码单个细胞毒性T淋巴细胞（CTL）表位的质粒DNA和编码卵清蛋白（OVA）的质粒DNA，我们比较了基因枪和肌肉注射免疫诱导免疫应答的重复性。与肌内接种相比，基因枪DNA免疫似乎在诱导针对CTL表位的特异性CTL和IFN-γ产生以及产生抗OVA IgG方面带来高度可重复和可靠的结果。肌肉接种的结果差异很大。我们的数据表明，皮肤基因枪免疫是一种更为可靠的DNA疫苗接种方法，可在动物模型中诱导有效的免疫应答。(C)2000爱思唯尔科技有限公司版权所有。
Utilizing a plasmid DNA encoding a single cytotoxic T lymphocyte (CTL) epitope and that encoding ovalbumin (OVA), we compared the reproducibility in the induction of immune responses by gene gun and intramuscular immunization. As compared to intramuscular inoculation, gene gun DNA immunization appeared to bring about highly reproducible and reliable results in the induction of specific CTL and IFN-gamma production to the CTL epitope and production of anti-OVA IgG. The results obtained by intramuscular inoculation vary significantly. Our data shown here strongly suggest that gene gun immunization of skin is a much more reliable method for DNA vaccination to induce effective immune responses in an animal model. (C) 2000 Elsevier Science Ltd. All rights reserved.