High pathogenicity and strong immunogenicity of a Chinese isolate of Eimeria magna Perard, 1925
High pathogenicity and strong immunogenicity of a Chinese isolate of Eimeria magna Perard, 1925
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中国分离的大艾美耳球虫 Perard 的高致病性和强免疫原性，1925。
DOI：
10.1016/j.parint.2017.01.014
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发表时间：
2017-06-01
影响因子：
1.9
通讯作者：
Liu, Xianyong
中科院分区：
文献类型：
--
作者：
Tao, Geru;Wang, Yunzhou;Liu, Xianyong
球虫感染一种或几种艾美耳球虫属寄生虫的兔子会引起球虫病，这种疾病会给兔子产业造成巨大的经济损失。大艾美耳球虫是兔球虫病的病原之一，在以往的研究中被定性为轻度致病性和中度免疫原性。在这项研究中，我们通过检测其生物学特征（卵囊形态、大小、专利前时间）和测序其内部转录间隔器1 (its -1) DNA片段，鉴定了一个中国分离物。该分离物具有高致病性；感染1 × 10(2)个卵囊的家兔在14天内体重增加减少55%。此外，1 × 10(2)个卵囊免疫可防止5 × 10(4)个卵囊再次感染时体重下降，表明该分离物具有较高的免疫原性。本研究描述了兔球虫中国分离株的独特表型，为兔球虫的地理变异研究做出了贡献。(C) 2017 Elsevier B.V.版权所有
Coccidia infection of rabbits with one or several species of parasites of the genus Eimeria causes coccidiosis, a disease leading to huge economic losses in the rabbit industry. Eimeria magna, one of the causal agents of rabbit coccidiosis, was characterized as mildly pathogenic and moderately immunogenic in previous studies. In this study, we identified a Chinese isolate of E. magna by testing its biological features (oocyst morphology and size, prepatent time) and sequencing its internal transcribed spacer 1 (ITS-1) DNA fragment. This isolate is highly pathogenic; infection of rabbits with only 1 x 10(2) oocysts caused a 55% reduction in weight gain in 14 days. In addition, immunization with 1 x 10(2) oocysts prevented body weight loss against re-infection with 5 x 10(4) oocysts, indicating the high immunogenicity of this isolate. Our study described the distinctive phenotype of the Chinese isolate of E. magna and contributed to the research of geographic variation of rabbit coccidia. (C) 2017 Elsevier B.V. All rights reserved.