A Novel Report of Apoptosis in Human Lung Carcinoma Cells Using Selective Agonist of D2-like Dopamine Receptors: A New Approach for the Treatment of Human Non-Small Cell Lung Cancer
A Novel Report of Apoptosis in Human Lung Carcinoma Cells Using Selective Agonist of D2-like Dopamine Receptors: A New Approach for the Treatment of Human Non-Small Cell Lung Cancer
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DOI：
10.1177/039463201302600212
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发表时间：
2013-04
影响因子：
3.5
通讯作者：
M. Sheikhpour;G. Ahangari;M. Sadeghizadeh;A. Deezagi
中科院分区：
文献类型：
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作者：
M. Sheikhpour;G. Ahangari;M. Sadeghizadeh;A. Deezagi
在我们先前的研究中，发现D2样多巴胺受体基因的低表达与非小细胞肺癌(NSCLC)疾病的关系。在这项新的研究中，通过使用这些受体的选择性激动剂溴隐亭(BR)，我们试图激活D2样蛋白的表达和诱导NSCLC选择性细胞的凋亡。此外，还研究了人肺癌细胞对BR和D2-多巴胺受体基因的凋亡反应的关系。将5种剂量的BR作用于人肺癌细胞(Qu-DB)48h，用四甲基偶氮唑盐(MTT)比色法测定细胞存活率。实时定量聚合酶链式反应(Real Time PCR)研究D2样多巴胺受体基因的表达模式。DAPI荧光染色观察细胞核形态，琼脂糖凝胶显示BR诱导DNA断裂。最后，用ANNECHIN-V-FUOS染色对细胞的凋亡及其与坏死的区别进行检测和定量。本研究证明BR对人肺癌细胞的增殖有抑制作用，并能诱导其发生凋亡。此外，D2-多巴胺受体基因的表达可能与细胞凋亡的发生发展有关。总之，BR负责诱导人肺癌细胞的凋亡，可用于治疗这些肿瘤细胞。此外，D2多巴胺受体的正常表达与BR对这些细胞的凋亡作用有关。
In our previous study, a relationship between low expression of D2-like dopamine receptor genes and non-small cell lung cancer (NSCLC) disease was found. In this new research, by using selective agonist of these receptors, Bromocriptine (BR), we attempted to activate D2-like expression and apoptotic induction in a selective cell line of NSCLC. In addition, the relationship of apoptotic response of human lung carcinoma cells to BR and D2- dopamine receptor genes is investigated. Human lung cancer (QU-DB) cells were treated by five doses of BR at 48 h and cell viability was determined by MTT assay. The gene expression pattern of D2-like dopamine receptor Genes was studied by Real Time PCR. Nuclear morphology of cells was monitored by DAPI florescent staining then induction of DNA fragmentation by BR was shown in an agarose gel. Finally, the detection and quantification of apoptosis and its differentiation from necrosis was carried out by using Annecxin-V-Fluos Staining. In this study, it is demonstrated that BR inhibited the proliferation of human lung cancer cells and induced apoptosis in them. In addition, the probable relationship between D2-dopamine receptor genes expression and the development of apoptosis was found. In conclusion, BR is responsible for induction of apoptosis in human lung cancer cells and can be used in treatment of these tumoric cells. In addition, normal expression of D2 dopamine receptors was associated with apoptotic effect of BR on these cells.