A pinch-valve for centrifugal microfluidic platforms and its application in sequential valving operation and plasma extraction
A pinch-valve for centrifugal microfluidic platforms and its application in sequential valving operation and plasma extraction
复制标题
DOI：
10.1016/j.snb.2015.06.034
复制
发表时间：
2015-12-31
影响因子：
8.4
通讯作者：
Wang, Wanjun
中科院分区：
文献类型：
--
作者：
Cai, Ziliang;Xiang, Jiwen;Wang, Wanjun
本文提出了一种适用于离心式微流控平台的新型针式阀。阀门系统的执行机构由一个弹簧柱塞和一个飞球调速器组成。该阀设计为“常闭”阀，通过使用弹簧柱塞对PDMS微通道施加预应力使其处于初始关闭状态。飞球总督驱动弹簧柱塞释放压缩时，它在一个特定的速度旋转打开阀门。该阀具有良好的鲁棒性，其爆破频率可以通过调节机械系统的参数来调节。在本文中，我们描述了一个单阀和两个顺序阀的操作。作为概念验证，开发了一种能够计量和离心沉淀的顺序阀系统，用于从全血中提取血浆。所得残留细胞浓度小于0.5%。这种新的阀门设计提供了一些主要的优势，在该领域的报告：简单的制造，强大的控制和易于集成。它在Lab-on-CD系统中的生物、化学和医学应用中表现出巨大的复杂流体处理潜力。(C)2015爱思唯尔B. V.保留所有权利。
This paper presents a new pin-type valve suitable for centrifugal microfluidic platforms. The actuator for the valving system consists of a spring plunger and a flyball governor. The valve is designed to be a "normally-closed" one by using a spring plunger to pre-stress the PDMS microchannel to an initially closed state. The flyball governor drives the spring plungers to release the compression when it spins at a particular speed to open the valve. The valve demonstrated robust performances and its burst frequency can be tuned by adjusting the parameters of the mechanical system. In this paper, we characterized the operation of one single valve and two sequential valves. As a proof of concept, a sequential valving system capable of metering and centrifugal sediment was developed for plasma extraction from whole blood. The resulting residual cell concentration was less than 0.5%. This new valve design offers some major advantages over those reported in the field: simple fabrication, robust control and easy integration. It demonstrates great potential for complex fluid handling needed in biological, chemical and medical applications in Lab-on-CD systems. (C) 2015 Elsevier B.V. All rights reserved.