Clear distinction of purine bases on the complementary strand by a fluorescence change of a novel fluorescent nucleoside
Clear distinction of purine bases on the complementary strand by a fluorescence change of a novel fluorescent nucleoside
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DOI：
10.1021/ja034090u
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发表时间：
2003-04-30
影响因子：
15
通讯作者：
Saito, I
中科院分区：
文献类型：
--
作者：
Okamoto, A;Tainaka, K;Saito, I
设计了一种新的荧光核苷-苯并吡啶并嘧啶（BPP），它能区分与BPP相对的A和G碱基。BPP的碱基配对简并性强烈地有助于急剧的荧光变化，这取决于与BPP相对的嘌呤碱基的类型。含有BPP的ODN探针与靶DNA的杂交有助于用肉眼判断位于靶DNA上特定位点的嘌呤碱基的类型。含BPP的ODN是一种非常有效的A/G SNP分型探针。
A new fluorescent nucleoside, benzopyridopyrimidine (BPP), which can sharply distinguish between A and G bases opposite BPP has been devised. The base-pairing degeneracy of BPP strongly contributes to the sharp fluorescence change that is dependent on the type of purine bases opposite BPP. The hybridization of an ODN probe containing BPP with a target DNA facilitates the judgment with the naked eye of the type of purine base located at a specific site on the target DNA. The BPP-containing ODN is a very effective probe for A/G SNP typing.