A novel splice variant of supervillin, SV5, promotes carcinoma cell proliferation and cell migration
A novel splice variant of supervillin, SV5, promotes carcinoma cell proliferation and cell migration
复制标题
超级绒毛蛋白SV5的新型剪接变体促进癌细胞增殖和细胞迁移
DOI：
10.1016/j.bbrc.2016.11.013
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发表时间：
2017-01-01
影响因子：
3.1
通讯作者：
Fang, Zhiyou
中科院分区：
文献类型：
--
作者：
Chen, Xueran;Yang, Haoran;Fang, Zhiyou
超绒毛蛋白是一种肌动蛋白相关蛋白，通过与肌球蛋白II、F-肌动蛋白和皮质蛋白相互作用来调节肌动蛋白动力学，以促进细胞收缩性和细胞运动性。在非肌肉细胞中已经报道了人超绒毛蛋白的两种剪接变体（SV 1和SV 4）; SV 1缺乏存在于较大同种型SV 4中的3个外显子。SV 2，也称为archvillin，存在于横纹肌中; SV 3，也称为平滑肌archvillin或SmAV，从平滑肌中克隆。在本研究中，我们确定了一个新的剪接变异体的超绒毛蛋白（SV 5）。SV 5包含一种新的剪接模式。在小鼠组织和细胞系中，SV 5主要在骨骼肌和心肌以及增殖细胞中表达，但在大多数正常组织中几乎检测不到。使用RNAi和拯救实验，我们在这里表明，SV 5显示改变癌细胞的功能特性，并调节细胞增殖和细胞迁移。(C)2016 Elsevier Inc. All rights reserved.
Supervillin is an actin-associated protein that regulates actin dynamics by interacting with Myosin II, F-actin, and Cortactin to promote cell contractility and cell motility. Two splicing variants of human Supervillin (SV1 and SV4) have been reported in non-muscle cells; SV1 lacks 3 exons present in the larger isoform SV4. SV2, also called archvillin, is present in striated muscle; SV3, also called smooth muscle archvillin or SmAV, was cloned from smooth muscle. In the present study, we identify a novel splicing variant of Supervillin (SV5). SV5 contains a new splicing pattern. In the mouse tissues and cell lines examined, SV5 was predominantly expressed in skeletal and cardiac muscles and in proliferating cells, but was virtually undetectable in most normal tissues. Using RNAi and rescue experiments, we show here that SV5 displays altered functional properties in cancer cells, and regulates cell proliferation and cell migration. (C) 2016 Elsevier Inc. All rights reserved.