Caulerpa lentillifera polysaccharides enhance the immunostimulatory activity in immunosuppressed mice in correlation with modulating gut microbiota
Caulerpa lentillifera polysaccharides enhance the immunostimulatory activity in immunosuppressed mice in correlation with modulating gut microbiota
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Caulerpa lentillifera 多糖增强免疫抑制小鼠的免疫刺激活性，与调节肠道微生物群相关
DOI：
10.1039/c9fo00713j
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发表时间：
2019
期刊：
影响因子：
6.1
通讯作者：
Chen Xuefeng
中科院分区：
文献类型：
--
作者：
Sun Yujiao;Liu Yang;Ai Chunqing;Song Shuang;Chen Xuefeng
本研究以环磷酰胺(CTX)诱导免疫抑制的BALB/c小鼠为模型，研究了豆腐果藻多糖(CLP)的免疫刺激活性。结果表明，该方能改善环磷酰胺所致免疫抑制小鼠体重、结肠长度和胸腺/脾指数的损伤，增加免疫抑制小鼠血清和胸腺、脾、结肠组织中白介素1β、肿瘤坏死因子α和超氧化物歧化酶的分泌。此外，CLP还能促进免疫抑制小鼠结肠组织分泌型免疫球蛋白A(SIgA)和粘蛋白2的产生。与上述免疫刺激作用相关的是，CLP对短链脂肪酸(SCFA)的产生和微生物区系的多样性和组成有积极的影响，如促进乳杆菌、芽孢杆菌科、瘤胃球菌科、梭状芽孢杆菌XVIII和螺杆菌的生长，而抑制类杆菌、巴氏杆菌和沙门氏菌的微生物种群。这些结果提示，CLP可调节免疫抑制小鼠的SCFA产生和肠道微生物区系，从而激发结肠粘膜免疫，从而激活血液、胸腺和脾的系统免疫。这些结果可能有助于理解CLP的功能，支持其作为新型益生素和免疫刺激剂的潜力。
In this study, the immunostimulatory activity of Caulerpa lentillifera polysaccharides (CLP) was elucidated in cytoxan (CTX)-induced immunosuppressed BALB/c mice. The results showed that CLP ameliorated the CTX-evoked damage to body weight, colon length and thymus/spleen indexes and enhanced the secretions of interleukin (IL)-1β, tumor necrosis factor (TNF)-α and superoxidase dismutase (SOD) in serum and thymic, splenic and colonic tissues of the immunosuppressed mice. Besides, CLP promoted the production of secretory immunoglobulin A (SIgA) and mucin2 in the colonic tissue of the immunosuppressed mice. Associated with the above immunostimulatory effects, CLP positively affected the production of short chain fatty acids (SCFAs) and microbiota diversity and composition, such as improvement in the growth of Lactobacillus, Coriobacteriaceae, Ruminococcaceae, Clostridium_XVIII and Helicobacter, whereas it suppressed the microbial populations of Bacteroides, Barnesiella and Lachnospiraceae. These findings suggested that CLP modulated SCFA production and gut microbiota in the immunosuppressed mice, evoking the colonic mucosal immunity, which might activate the systemic immunity in blood, thymus and spleen. The results could be helpful for understanding the functions of CLP, supporting their potential as novel prebiotics and immunostimulators.