Effects of temperature and volume on intraperitoneal saline-induced changes in blood pressure, nociception, and neural activity in the rostroventral medulla.
Effects of temperature and volume on intraperitoneal saline-induced changes in blood pressure, nociception, and neural activity in the rostroventral medulla.
复制标题
温度和体积对腹腔内盐水引起的血压、伤害感受和延髓前腹神经活动变化的影响。
DOI：
10.1016/s0006-8993(02)03135-9
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发表时间：
2002
期刊：
影响因子：
2.9
通讯作者：
Thurston-Stanfield,CindyL
中科院分区：
文献类型：
--
作者：
Thurston-Stanfield,CindyL
假设吻侧腹内侧髓质的ON细胞和OFF细胞调节痛觉，ON细胞促进疼痛，OFF细胞抑制疼痛。本研究分析了不同体积和温度的生理盐水对轻麻醉大鼠伤害感觉（轻甩尾反射）、血压和on、OFF细胞活性的影响。大体积（20cc /kg）时，室温生理盐水刺激7/11 ON细胞，抑制10/12 OFF细胞2-5分钟。相比之下，大体积（20 cc/kg）体温生理盐水（37℃）只激发了1/10个ON细胞，抑制了3/13个OFF细胞，小体积（1-2 cc/kg）室温生理盐水只激发了3/10个ON细胞，抑制了4/11个OFF细胞。大剂量生理盐水给药后，甩尾潜伏期增加，时间的影响显著，而温度的影响不显著。ON细胞的兴奋和OFF细胞的抑制表明冷盐水可能是疼痛的，甩尾潜伏期的增加可能表明弥漫性有害抑制控制。也有可能是ON和OFF细胞活性的变化导致痛觉过敏，而痛觉过敏被独立于ON和OFF细胞介导的同时性痛觉减退所掩盖。由于腹腔注射生理盐水可能产生疼痛或痛觉过敏，因此在实验或临床使用生理盐水时应谨慎使用。
ON and OFF cells of the rostral ventromedial medulla are hypothesized to modulate nociception with ON cells facilitating pain and OFF cells inhibiting pain. The current study analyzed the effects of intraperitoneal saline at different volumes and temperatures on nociception (tail flick reflex), blood pressure, and the activity of ON and OFF cells in lightly anesthetized rats. At large volumes (20 cc/kg), room temperature saline excited 7/11 ON cells and inhibited 10/12 OFF cells for 2–5 min. In contrast, large volumes (20 cc/kg) of body temperature saline (37°C) excited only 1/10 ON cells and inhibited only 3/13 OFF cells, and small volumes (1–2 cc/kg) of room temperature saline excited only 3/10 ON cells and inhibited only 4/11 OFF cells. Tail flick latency increased following saline administration at large volumes with a significant effect of time, but not temperature. The excitation of ON cells and inhibition of OFF cells indicate that cold intraperitoneal saline could be painful and the increase in tail flick latency may indicate a diffuse noxious inhibitory control. It is also possible that the changes in ON and OFF cell activity caused a hyperalgesia that was masked by a simultaneous hypoalgesia that was mediated independent of the ON and OFF cells. Because intraperitoneal saline may produce pain or hyperalgesia, care should be used when saline is used experimentally or clinically.