Galactofuranose-Coated Gold Nanoparticles Elicit a Pro-inflammatory Response in Human Monocyte-Derived Dendritic Cells and Are Recognized by DC-SIGN
Galactofuranose-Coated Gold Nanoparticles Elicit a Pro-inflammatory Response in Human Monocyte-Derived Dendritic Cells and Are Recognized by DC-SIGN
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DOI：
10.1021/cb4008265
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发表时间：
2014-02-01
影响因子：
4
通讯作者：
Tefsen, Boris
中科院分区：
文献类型：
--
作者：
Chiodo, Fabrizio;Marradi, Marco;Tefsen, Boris
呋喃半乳糖（Galf）是一种五元环状半乳糖，仅存在于非哺乳动物物种中，其中一些是病原体。为了确定这种碳水化合物在宿主病原体相互作用中的假定作用，我们合成了携带Galf的多价金纳米颗粒（Galf-GNPs），并表明它们可以被EB-A2抗体识别，EB-A2抗体广泛用于检测血清中含有Galf的半乳甘露聚糖。曲霉病患者。我们证明了人单核细胞来源的树突状细胞通过与凝集素DC-SIGN的相互作用结合Galf-GNP。此外，树突状细胞与Galf-GNP的相互作用导致这些细胞上几种成熟标志物的表达增加，并诱导促炎细胞因子IL-6和TNF-α的分泌。这些数据表明Galf能够通过树突状细胞调节先天免疫应答。总之，Galf-GNP是一种通用的工具，可以应用于多功能研究，以更好地了解Galf在宿主-病原体相互作用中的作用。
Galactofuranose (Galf) is the five-membered ring form of galactose exclusively found in nonmammalian species, among which several are pathogens. To determine the putative role of this carbohydrate in host pathogen interactions, we synthesized multivalent gold nanoparticles carrying Galf (Galf-GNPs) and show that they are recognized by the EB-A2 antibody, which is widely used to detect Galf-containing galactomannan in the serum of Aspergillosis patients. We demonstrated that human monocyte-derived dendritic cells bound Galf-GNPs via interaction with the lectin DC-SIGN. Moreover, interaction of dendritic cells with Galf-GNPs resulted in increased expression of several maturation markers on these cells and induced secretion of the pro-inflammatory cytokines IL-6 and TNF-alpha. These data indicate that Galf is able to modulate the innate immune response via dendritic cells. In conclusion, Galf-GNPs are a versatile tool that can be applied in multiple functional studies to gain a better understanding of the role of Galf in host-pathogen interaction.