Analysis of the interaction of primate retroviruses with the human RNA interference machinery
Analysis of the interaction of primate retroviruses with the human RNA interference machinery
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DOI：
10.1128/jvi.01390-07
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发表时间：
2007-11-01
影响因子：
5.4
通讯作者：
Cullen, Bryan R.
中科院分区：
文献类型：
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作者：
Lin, Jennifer;Cullen, Bryan R.
逆转录病毒，包括人类免疫缺陷病毒I型（HIV-1），是否与细胞RNA干扰机制相互作用的问题一直存在争议。在这里，我们提供的数据表明，无论是HIV-1还是人类T细胞白血病病毒1型（HTLV-1）在持续感染的T细胞中表达显着水平的小干扰RNA或microRNA。我们还证明，逆转录病毒核转录因子HIV-1达特和HTLV-1 Tax，以及由灵长类泡沫病毒编码的Tas反式激活因子，不能抑制人类细胞中的RNA干扰。此外，HIV-1达特的生理水平的稳定表达不会全面抑制受感染的人类细胞中微小RNA的产生或表达。总之，这些数据表明，HIV-1和HTLV-1既不诱导病毒小干扰RNA或microRNA的产生，也不抑制感染细胞中的细胞RNA干扰机制。
The question of whether retroviruses, including human immunodeficiency virus type I (HIV-1), interact with the cellular RNA interference machinery has been controversial. Here, we present data showing that neither HIV-1 nor human T-cell leukemia virus type 1 (HTLV-1) expresses significant levels of either small interfering RNAs or microRNAs in persistently infected T cells. We also demonstrate that the retroviral nuclear transcription factors HIV-1 Tat and HTLV-1 Tax, as well as the Tas transactivator encoded by primate foamy virus, fail to inhibit RNA interference in human cells. Moreover, the stable expression of physiological levels of HIV-1 Tat did not globally inhibit microRNA production or expression in infected human cells. Together, these data argue that HIV-1 and HTLV-1 neither induce the production of viral small interfering RNAs or microRNAs nor repress the cellular RNA interference machinery in infected cells.