Detection of Mycoplasma pneumoniae by loop-mediated isothermal amplification (LAMP) assay and serology in pediatric community-acquired pneumonia
Detection of Mycoplasma pneumoniae by loop-mediated isothermal amplification (LAMP) assay and serology in pediatric community-acquired pneumonia
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DOI：
10.1007/s10156-012-0388-5
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发表时间：
2012-10-01
影响因子：
2.2
通讯作者：
Ozaki, Takao
中科院分区：
文献类型：
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作者：
Gotoh, Kensei;Nishimura, Naoko;Ozaki, Takao
肺炎支原体肺炎的快速诊断需要及时有效的抗菌药物治疗;然而，这种能力尚未在临床实践中建立。近年来，一种新的核酸扩增方法，称为环介导等温扩增（LAMP）已被用于快速诊断各种传染病。在这项研究中，我们前瞻性地评估了LAMP检测快速诊断M。肺炎的临床实践。本研究纳入了2009年4月至2010年3月期间因社区获得性肺炎住院的368名儿童（中位年龄3.8岁;范围0.1-14.3岁）。我们在入院时采集咽拭子检测M。在入院时和出院时将肺炎链球菌DNA和配对血清样品用于测定M。肺炎抗体滴度。M.通过阳性LAMP测定或抗体滴度增加四倍或更多来诊断肺炎性肺炎。总体而言，46名儿童（占肺炎患者的12.5%）被诊断为M。肺炎性肺炎;其中，27例（58.7%）年龄小于6岁。在上述46名儿童中，LAMP和血清学分别确定了38名（82.6%）和37名（80.4%）。以血清学检测结果为标准，LAMP法的敏感性为78.4%，特异性为97.3%，阳性预测值为76.3%，阴性预测值为97.6%。结论：LAMP法可用于M.肺炎
Rapid diagnosis of Mycoplasma pneumoniae pneumonia is required for treatment with effective antimicrobial agents without delay; however, this capacity has not yet been established in clinical practice. Recently, a novel nucleic acid amplification method termed loop-mediated isothermal amplification (LAMP) has been used to rapidly diagnose various infectious diseases. In this study, we prospectively evaluated the efficacy of the LAMP assay to rapidly diagnose M. pneumoniae pneumonia in clinical practice. Three hundred sixty-eight children (median age, 3.8 years; range, 0.1-14.3 years) admitted to our hospital between April 2009 and March 2010 for community-acquired pneumonia were enrolled in this study. We obtained throat swabs on admission to detect M. pneumoniae DNA and paired serum samples on admission and at discharge to assay M. pneumoniae antibody titers. M. pneumoniae pneumonia was diagnosed by either a positive LAMP assay or a fourfold or greater increase in antibody titer. Overall, 46 children (12.5% of the patients with pneumonia) were diagnosed with M. pneumoniae pneumonia; of these, 27 (58.7%) were aged less than 6 years. Of the aforementioned 46 children, 38 (82.6%) and 37 (80.4%) were identified by LAMP and serology, respectively. When the results of serology were taken as the standard, the sensitivity and specificity and positive and negative predictive values of the LAMP assay were 78.4%, 97.3%, 76.3%, and 97.6%, respectively. We concluded the LAMP assay may be useful for rapid diagnosis of M. pneumoniae pneumonia.