Towards a protein interaction map of potyviruses:: protein interaction matrixes of two potyviruses based on the yeast two-hybrid system
Towards a protein interaction map of potyviruses:: protein interaction matrixes of two potyviruses based on the yeast two-hybrid system
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DOI：
10.1099/0022-1317-82-4-935
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发表时间：
2001-04-01
影响因子：
3.8
通讯作者：
Valkonen, JPT
中科院分区：
文献类型：
--
作者：
Guo, DY;Rajamäki, ML;Valkonen, JPT
马铃薯A病毒（PVA）和豌豆种传花叶病毒（PSbMV）主要蛋白质互作图谱的构建利用酵母双杂交系统（YTHS）获得了马铃薯Y病毒属（Potyviridae）的5种PVA蛋白组合，在另外7种组合中检测到了HC-HC、HC-CI、VPg-VPg、Nla-Nlb和CP-CP之间的相互作用和弱但可重复的相互作用在PSbMV中，在五种蛋白质组合中发现了容易检测到的相互作用（P1-Cl、P3-Nlb、NlaPro-Nlb、VPg-Nla、VPg-NlaPro、NlaPro-Nla和Nla-Nla）。对于另外三种组合，检测到HC-HC、VPg-VPg、VPg-Nla、Nla-Nla和Nla-Nlb）和较弱但可重复的相互作用因此，HC、VPg、Nla和CP的自身相互作用以及VPg-Nla、Nla-NlaPro和Nla-Nlb的相互作用对于两种马铃薯Y病毒是共同的，这项研究揭示的多种蛋白质相互作用揭示了协同作用，马铃薯Y病毒蛋白参与病毒运动和复制的协调功能。
A map for the interactions of the major proteins from Potato virus A (PVA) and Pea seed-borne mosaic Virus (PSbMV) (members of the genus Potyvirus, family Potyviridae) was generated using the yeast two-hybrid system (YTHS), Interactions were readily detected with five PVA protein combinations (HC-HC, HC-CI, VPg-VPg, Nla-Nlb and CP-CP) and weak but reproducible interactions were detected for seven additional combinations (P 1-CI, P3-Nlb, NlaPro-Nlb, VPg-Nla, VPg-NlaPro, NlaPro-Nla and Nla-Nla), In PSbMV, readily detectable interactions were found in five protein combinations (HC-HC, VPg-VPg, VPg-Nla, Nla-Nla and Nla-Nlb) and weaker but reproducible interactions were detected for three additional combinations (P3-Nla, Nla-NlaPro and CP-CP), The self-interactions of HC, VPg, Nla and CP and the interactions of VPg-Nla, Nla-NlaPro and Nla-Nlb were, therefore, common for the two potyviruses, The multiple protein interactions revealed in this study shed light on the co-ordinated functions of potyviral proteins involved in virus movement and replication.