Improved chemical pulping and saccharification of a natural mulberry mutant deficient in cinnamyl alcohol dehydrogenase
Improved chemical pulping and saccharification of a natural mulberry mutant deficient in cinnamyl alcohol dehydrogenase
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DOI：
10.1515/hf-2021-0015
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发表时间：
2021-05
期刊：
影响因子：
2.4
通讯作者：
Tsutomu Ikeda;N. Takata;Shingo Sakamoto;Shi Hu;Nuoendagula;S. Hishiyama;Nobutaka Mitsuda;W. Boerjan;J. Ralph;S. Kajita
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文献类型：
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作者：
Tsutomu Ikeda;N. Takata;Shingo Sakamoto;Shi Hu;Nuoendagula;S. Hishiyama;Nobutaka Mitsuda;W. Boerjan;J. Ralph;S. Kajita
摘要木质素含量及其分子结构影响木材的各种特性。在这项研究中，木材的解剖学和物理化学性质来自一个自然发生的桑树突变体缺乏肉桂醇脱氢酶（CAD），在木质素生物合成的关键酶，进行了分析，使用常规的染色测定干切片，木质部纤维细胞的长度和宽度的测量，木材制浆和糖化测定，和糖成分分析的提取物自由木粉。目前的数据表明，CAD基因中的突变导致改进的木材脱木质素效率，在碱性制浆条件下增加的纸浆产率，以及在碱预处理后增强的糖化效率。这项研究为桑树CAD缺陷突变体作为生物精炼工艺的原料的多用途用途开辟了新的途径，除了其作为蚕的传统用途外。
Abstract Lignin content and its molecular structure influence various wood characteristics. In this study, the anatomical and physicochemical properties of wood derived from a naturally occurring mulberry mutant deficient in cinnamyl alcohol dehydrogenase (CAD), a key enzyme in lignin biosynthesis, were analyzed using conventional staining assays on stem sections, length and width measurements of xylem fiber cells, wood pulping and saccharification assays, and sugar compositional analysis of extractive-free wood powder. The present data indicate that the mutation in the CAD gene leads to improved wood delignification efficiency, increased pulp yield under alkaline pulping conditions, and enhanced saccharification efficiency following alkaline pretreatment. This study opens up new avenues for the multipurpose use of the mulberry CAD-deficient mutant as a raw material for biorefinery processes, in addition to its traditional use as a favored feed for silkworms.