EXAMINATION OF STRATUM-CORNEUM BARRIER FUNCTION INVIVO BY INFRARED-SPECTROSCOPY
EXAMINATION OF STRATUM-CORNEUM BARRIER FUNCTION INVIVO BY INFRARED-SPECTROSCOPY
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DOI：
10.1111/1523-1747.ep12555503
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发表时间：
1990-10-01
影响因子：
6.5
通讯作者：
GUY, RH
中科院分区：
文献类型：
--
作者：
BOMMANNAN, D;POTTS, RO;GUY, RH
一般认为角质层（SC）是表皮的最低渗透层。然而，在组织学上，SC是不均匀的，不均匀的膜，并且问题“屏障功能是否均匀地分布在SC厚度上？“被摆了姿势为了解决这一问题，人腹侧前臂SC在体内进行了研究，在连续磁带剥离过程中的衰减全反射傅里叶变换红外光谱。由于SC的细胞间脂质和膜的水化程度已被证明是屏障功能的关键决定因素，因此注意力一直集中在光谱特征上，这些特征具体报告这些参数。已发现SC细胞间脂质的紊乱程度在外细胞层（多达三个条带）上降低，然后保持基本恒定。脂质的量类似地减少，使得在约四次胶带剥离后观察到60%的减少（相对于“无剥离”基线）。对这些测量结果的一个合理解释是，表面附近的脂质是（a）“真正的”细胞间脂质（预计是高度有序的）和（B）皮脂脂质（含有大量的低熔点组分，如脂肪酸）的混合物。连续的红外（IR）光谱至少提供了间接证据来支持这一假设。正如预期的那样，红外光谱表明，SC水合作用从表面向SC-颗粒层界面增加。两者合计，结果表明，SC确实是不均匀的。外层的性质（由前3-4个胶带剥离去除的那些）随着深度的增加而显著变化。随后进入的层（由胶带5至14所揭示的层）意味着屏障的该较深部分在其整个厚度上相对一致，没有证据表明存在单一的、特别耐腐蚀的组分。
It is generally accepted that the stratum corneum (SC) is the least permeable layer of the epidermis. Histologically, though, the SC is a non-uniform, inhomogeneous membrane, and the question "Is barrier function distributed uniformly across the SC thickness?" has been posed. To address this issue, human ventral forearm SC has been studied in vivo by attenuated-total-reflectance Fourier-transform infrared spectroscopy during the course of sequential tape-stripping. Because the intercellular lipids of the SC and the degree of hydration of the membrane have been shown to be crucial determinants of barrier function, attention has been focused on the spectral features, which report specifically on these parameters. The degree of disorder of the SC intercellular lipids has been found to decrease over the outer cell layers (up to three tape-strips) and then to remain essentially constant. The amount of lipids decreases similarly such that a 60% reduction (relative to the "no-strip" baseline) is observed after about four tape-strips. A plausible explanation for these measurement is that the lipids near the surface are a mixture of (a) "true" intercellular lipid (which is expected to be highly ordered), and (b) sebaceous lipid (which contains much greater amounts of low-melting components, such as fatty acids). The sequential infrared (IR) spectra provide at least circumstantial evidence to support this hypothesis. As expected, the IR spectra show that SC hydration increases from the surface towards the SC-stratum granulosum interface. Taken together, the results imply that the SC is indeed non-uniform. The properties of the outer layers (those removed by the first 3-4 tape-strips) change significantly with increasing depth. Subsequently accessed layers (those revealed by tape-strips 5 through 14) imply that this deeper part of the barrier is relatively consistent throughout its thickness, with no evidence for a single, particularly resistant, component.