Fgf16 is essential for pectoral fin bud formation in zebrafish
Fgf16 is essential for pectoral fin bud formation in zebrafish
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DOI：
10.1016/j.bbrc.2006.06.108
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发表时间：
2006-08-18
影响因子：
3.1
通讯作者：
Itoh, Nobuyuki
中科院分区：
文献类型：
--
作者：
Nomura, Ryohei;Kamei, Eriko;Itoh, Nobuyuki
斑马鱼胸鳍芽的形成是研究形态发生的一个很好的模型。成纤维细胞生长因子（Fgfs）和音刺猬（shh）是胸鳍芽形成所必需的。我们发现Fgf16在鳍芽的顶端外胚层嵴（AER）中表达。Fgf16功能的敲低导致没有鳍芽生长。Fgf16分别是鳍芽间充质细胞增殖和分化以及AER所必需的。Fgf16在Fgf10（一种间充质因子）的下游起作用，其信号传导以诱导Fgf4和Fgf8在AER中的表达。AER中的Fgf 16和极化活动区（ZPA）中的shh相互作用以诱导和/或维持彼此的表达。这些结果表明，Fgf16，一个新发现的AER因子，通过介导AER-间充质和AER-ZPA的相互作用，在胸鳍芽的生长中起着至关重要的作用。(c)2006年爱思唯尔公司All rights reserved.
Zebrafish pectoral fin bud formation is an excellent model for studying morphogenesis. Fibroblast growth factors (Fgfs) and sonic hedgehog (shh) are essential for pectoral fin bud formation. We found that Fgf16 was expressed in the apical ectodermal ridge (AER) of fin buds. A knockdown of Fgf16 function resulted in no fin bud outgrowth. Fgf16 is required for cell proliferation and differentiation in the mesenchyme and the AER of the fin buds, respectively. Fgf16 functions downstream of Fgf10, a mesenchymal factor, signaling to induce the expression of Fgf4 and Fgf8 in the AER. Fgf16 in the AER and shh in the zone of polarizing activity (ZPA) interact to induce and/or maintain each other's expression. These findings have revealed that Fgf16, a newly identified AER factor, plays a crucial role in pectoral fin bud outgrowth by mediating the interactions of AER-mesenchyme and AER-ZPA. (c) 2006 Elsevier Inc. All rights reserved.