Liberation of a fibrogenic factor from human blood monocytes, ascites cells, cultured histiocytes and transformed mouse macrophages by treatment with SiO2.
Liberation of a fibrogenic factor from human blood monocytes, ascites cells, cultured histiocytes and transformed mouse macrophages by treatment with SiO2.
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通过 SiO2 处理，从人血单核细胞、腹水细胞、培养的组织细胞和转化的小鼠巨噬细胞中释放纤维形成因子。
DOI：
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发表时间：
1980
影响因子：
2.1
通讯作者：
J. Vilpo
中科院分区：
文献类型：
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作者：
M. Aalto;E. Kulonen;T. Rönnemaa;C. Sundström;J. Vilpo
在Percoll梯度中分离来自白血病患者的人单核细胞和腹水巨噬细胞，并在有和没有二氧化硅的情况下培养。还用培养的恶性人组织细胞和转化的小鼠巨噬细胞进行了类似的实验。通过测量[3H]脯氨酸和[3H]胸苷掺入培养的大鼠肉芽肿和人滑膜细胞来测试培养基的纤维化活性。从二氧化硅处理的单核细胞，腹水巨噬细胞和某些组织细胞和小鼠巨噬细胞系的媒体引起的增加，[3H]脯氨酸和[3H]胸苷掺入胶原和DNA，分别在这两个细胞系统。碱性核糖核酸酶活性显着下降，从二氧化硅处理的腹水巨噬细胞的媒体，但不是在媒体的单核细胞或组织细胞。
Human monocytes and ascites macrophages from cirrhotic patients were isolated in Percoll-gradient and cultured with and without silica. Similar experiments were carried out also with cultured malignant human histiocytes and transformed mouse macrophages. The fibrogenic activity of the culture media was tested by measuring the incorporation of [3H]proline and [3H]thymidine into cultured rat granuloma and human synovial cells. Media from silica-treated monocytes, ascites macrophages and certain histiocyte and mouse macrophage lines caused an increase in the incorporation of both [3H]proline and [3H]thymidine into collagen and DNA, respectively, in both cell systems. Alkaline RNase activities were decreased markedly in the media from silica-treated ascites macrophages but not in the media of the monocytes or histiocytes.