Candida albicans CaPTC6 is a functional homologue for Saccharomyces cerevisiae ScPTC6 and encodes a type 2C protein phosphatase
Candida albicans CaPTC6 is a functional homologue for Saccharomyces cerevisiae ScPTC6 and encodes a type 2C protein phosphatase
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白色念珠菌 CaPTC6 是酿酒酵母 ScPTC6 的功能同源物，编码 2C 型蛋白磷酸酶
作者：
Jiang, Yuanying;Cao, Yongbing;Jiang, Linghuo;Zhao, Jingwen;Feng, Jinrong;Yu, Liquan;Fang, Jiaping;Feng, Chen
2C型蛋白磷酸酶（PP2C）是单体酶，其活性需要镁或锰离子的存在。酿酒酵母中有7个PP2C基因，即ScPTC 1、ScPTC 2、ScPTC 3、ScPTC 4、ScPTC 5、ScPTC 6和ScPTC 7。PTC6在致病性和非致病性酵母中高度保守。在本研究中，我们已经证明白色念珠菌CaPTC 6基因可以补充ScPTC 6在雷帕霉素和咖啡因敏感性S.酿酒酵母细胞，表明它们是功能同源物。我们还证明了CaPtc6编码的蛋白质是典型的PP2C酶，并且CaPtc6p定位于酵母形式和菌丝细胞的菌丝体中。然而，CaPTC 6的缺失既不影响细胞和菌丝生长，也不使念珠菌细胞对雷帕霉素和咖啡因敏感。因此，可能与其他线粒体磷酸酶的功能冗余，CaPtc6p可能参与线粒体生理的调节。版权所有© 2009约翰威利父子有限公司。
Type 2C protein phosphatases (PP2C) are monomeric enzymes and their activities require the presence of magnesium or manganese ions. There are seven PP2C genes, ScPTC1, ScPTC2, ScPTC3, ScPTC4, ScPTC5, ScPTC6 and ScPTC7, in Saccharomyces cerevisiae. PTC6 is highly conserved in pathogenic and nonpathogenic yeasts. In the current study we have demonstrated that the Candida albicans CaPTC6 gene could complement the functions of ScPTC6 in the rapamycin and caffeine sensitivities of S. cerevisiae cells, indicating that they are functional homologues. We have also demonstrated that the CaPTC6‐encoded protein is a typical PP2C enzyme and that CaPtc6p is localized in the mitochondrion of yeast‐form and hyphal cells. However, deletion of CaPTC6 neither affects cell and hyphal growth nor renders Candida cells sensitive to rapamycin and caffeine. Therefore, possibly with a functional redundancy to other mitochondrial phosphatases, CaPtc6p is likely to be involved in the regulation of a mitochondrial physiology. Copyright © 2009 John Wiley & Sons, Ltd.
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DOI：
10.1016/s0021-9258(18)54124-7
发表时间：
1993-01
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kohji Fukunaga;Takayasu Kobayashi;S. Tamura;E. Miyamoto
通讯作者：
Kohji Fukunaga;Takayasu Kobayashi;S. Tamura;E. Miyamoto
影响因子：
9.8
作者：
Park CJ;Peng Y;Chen X;Dardick C;Ruan D;Bart R;Canlas PE;Ronald PC
通讯作者：
Ronald PC
DOI：
--
发表时间：
1989-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
P. Cohen;P. Cohen
通讯作者：
P. Cohen;P. Cohen
影响因子：
5.3
作者：
A. Welihinda;W. Tirasophon;S. R. Green;R. Kaufman
通讯作者：
A. Welihinda;W. Tirasophon;S. R. Green;R. Kaufman
影响因子：
5.7
作者：
S. Tamura;Shinnosuke Toriumi;J. Saito;K. Awano;Tada-Aki Kudo;Takayasu Kobayashi
通讯作者：
S. Tamura;Shinnosuke Toriumi;J. Saito;K. Awano;Tada-Aki Kudo;Takayasu Kobayashi