IDENTIFICATION OF STRUCTURAL GENE FOR MYOSIN HEAVY-CHAIN IN CAENORHABDITIS-ELEGANS
IDENTIFICATION OF STRUCTURAL GENE FOR MYOSIN HEAVY-CHAIN IN CAENORHABDITIS-ELEGANS
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DOI：
10.1016/0022-2836(77)90287-x
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发表时间：
1977-01-01
影响因子：
5.6
通讯作者：
BRENNER, S
中科院分区：
文献类型：
--
作者：
MACLEOD, AR;WATERSTON, RH;BRENNER, S
用氰化和十二烷基硫酸钠/聚丙烯酰胺凝胶电泳法对线虫UNC-54基因突变株的肌球蛋白进行鉴定。在缺乏总肌球蛋白主要部分的隐性突变体中，高相对分子质量倍体为15倍。104和14倍。104，这是野生型肌球蛋白的主要氰化模式。在突变体E675中，具有一条新的重链，其相对分子质量为2倍。105，氰化二联体的每个组分减少104道尔顿，表明该二联体是由单个多肽链的部分切割而来的。UNC-54显然是肌球蛋白重链的结构基因，肌球蛋白重链存在于线虫总肌球蛋白的主要部分。
Mutants in the unc-54 gene of C. elegans were characterized by cyanylation and sodium dodecyl sulfate/polyacrylamide gel electrophoresis of the total myosin present in each mutant. In the recessive mutants lacking a major fraction of the total myosin, the high MW doublet of 15 .times. 104 and 14 .times. 104 which dominates the cyanylation pattern of the total wild-type myosin is absent. In the mutant E675, which possesses a novel heavy-chain with a MW of 2 .times. 105, each component of the cyanylation doublet is reduced by 104 daltons, indicating that the doublet is derived from partial cleavage of a single polypeptide chain. The unc-54 is apparently the structural gene for a myosin heavy-chain present in a major fraction of the total nematode myosin.