DNA transposition of bacteriophage Mu -: A quantitative analysis of target site selection in vitro
DNA transposition of bacteriophage Mu -: A quantitative analysis of target site selection in vitro
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DOI：
10.1074/jbc.m108044200
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发表时间：
2002-01-25
影响因子：
4.8
通讯作者：
Savilahti, H
中科院分区：
文献类型：
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作者：
Haapa-Paananen, S;Rita, H;Savilahti, H
Mu转位DNA重组机制通过组装与目标DNA相互作用的蛋白质-DNA复合物来选择目标位点，并在定位有利的序列组成时发生反应。将转座子剪接到靶标上会产生一个5bp的复制，反映了原始靶标位点。利用最小Mu体外系统考察了不同目标五聚体的优先使用情况，并对最优先位点和最不优先位点进行了定量编译。当作为基本步骤进行分析时，检测到沿5-bp目标位点的某些步骤的偏好。我们进一步证明，插入位置可以在加性计算的基本步长值的基础上预测。此外，周围序列也影响给定五聚体的偏好；一个对称的结构组件被揭示出来，表明潜在的铰链在目标部位和周围。
The Mu transpositional DNA recombination machinery selects target sites by assembling a protein-DNA complex that interacts with the target DNA and reacts whenever it locates a favorable sequence composition. Splicing of a transposon into the target generates a 5-bp duplication that reflects the original target site. Preferential usage of different target pentamers was examined with a minimal Mu in vitro system and quantitatively compiled consensus sequences for the most preferred and the least preferred sites were generated. When analyzed as base steps, preferences toward certain steps along the 5-bp target site were detected. We further show that insertion sites can be predicted on the basis of additively calculated base step values. Also surrounding sequences influence the preference of a given pentamer; a symmetrical structural component was revealed, suggesting potential hinges at and around the target site.