Isolation and characterization of a Drosophila gene that encodes multiple neuropeptides related to Phe-Met-Arg-Phe-NH2 (FMRFamide).
Isolation and characterization of a Drosophila gene that encodes multiple neuropeptides related to Phe-Met-Arg-Phe-NH2 (FMRFamide).
复制标题
DOI：
10.1073/pnas.85.6.1993
复制
发表时间：
1988-03
影响因子：
11.1
通讯作者：
L. E. Schneider;P. Taghert
中科院分区：
文献类型：
--
作者：
L. E. Schneider;P. Taghert
通过用果蝇Phe-Met-Arg-Phe-NH_2 cDNA片段和合成的寡核苷酸筛选基因组文库，分离到一个编码与软体动物Phe-Met-Arg-Phe-NH_2相关的神经肽（FMRFamide）的果蝇基因。该基因用于从果蝇成虫头部cDNA文库中分离cDNA。通过序列分析确定该cDNA编码13个在其羧基端具有Phe-Met-Arg-Phe-NH 2或相关序列的肽。其他推定的神经肽，包括一个具有同源性的哺乳动物促肾上腺皮质激素释放因子，是目前在推导约等于39 kDa的前体。Southern杂交分析表明，在单倍体基因组中存在一个单一的Phe-Met-Arg-Phe-NH 2-like基因。RNA印迹分析表明至少两种转录物的表达约等于1.7和约等于0.7个酶。这两个成绩单是显而易见的整个幼虫，蛹，和成人的发展阶段。用原位杂交技术将该基因定位于第2号染色体右臂46 C带。这些数据为利用果蝇的先进遗传学和分子技术来解决神经肽表达和功能的复杂方面提供了基础。
A Drosophila gene that encodes neuropeptides related to molluscan Phe-Met-Arg-Phe-NH2 (FMRFamide) was isolated by screening a genomic library with a fragment of an Aplysia Phe-Met-Arg-Phe-NH2 cDNA and with synthetic oligonucleotides. This gene was used to isolate a cDNA from a Drosophila adult head cDNA library. The cDNA was defined by sequence analysis to encode 13 peptides that have Phe-Met-Arg-Phe-NH2 or related sequences at their carboxyl termini. Other putative neuropeptides, including one that has homology to mammalian corticotropin-releasing factor, are present in the deduced approximately equal to 39-kDa precursor. Southern blot analysis suggested the presence of a single Phe-Met-Arg-Phe-NH2-like gene within the haploid genome. RNA blot analysis indicated the expression of at least two transcripts of approximately equal to 1.7 and approximately equal to 0.7 kilobases. Both transcripts are evident throughout larval, pupal, and adult developmental stages. In situ hybridization was used to localize this neuropeptide gene to band 46C on the right arm of the 2nd chromosome. These data provide the basis for utilizing the advanced genetics and molecular techniques of Drosophila to address complex aspects of neuropeptide expression and function.