Binding of NCK to SOS and activation of ras-dependent gene expression.
Binding of NCK to SOS and activation of ras-dependent gene expression.
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NCK 与 SOS 的结合以及 ras 依赖性基因表达的激活。
DOI：
10.1128/mcb.15.3.1169
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发表时间：
1995
影响因子：
5.3
通讯作者：
Williams,LT
中科院分区：
文献类型：
--
作者：
Hu,Q;Milfay,D;Williams,LT
NCK是一种含有SH 2和SH 3结构域的蛋白质，在生长因子刺激下磷酸化并与酪氨酸激酶受体相关。NCK的序列表明，NCK作为受体和下游信号分子之间的接头发挥作用。为了确定NCK是否可以介导生长因子刺激的反应，我们测量了NCK激活thefos启动子的能力。我们发现，在NIH 3 T3细胞中，NCK强烈激活该启动子。NCK对thefos启动子的作用被c-ras增强，被显性负调控因子阻断。我们还发现，NCK直接结合到鸟嘌呤核苷酸交换因子SOS。这种相互作用由NCK的SH 3结构域介导。这些结果表明，NCK可以通过与SOS蛋白的相互作用来调节p21 ras依赖的基因转录。
NCK, an SH2- and SH3 domain-containing protein, becomes phosphorylated and associated with tyrosine kinase receptors upon growth factor stimulation. The sequence of NCK suggests that NCK functions as a linker between receptors and a downstream signaling molecule. To determine if NCK can mediate growth factor-stimulated responses, we measured the ability of NCK to activate thefospromoter. We found that in NIH 3T3 cells, NCK strongly activates this promoter. The effect of NCK on thefospromoter is enhanced by c-rasand blocked by dominant negativeras. We also found that NCK binds directly to the guanine nucleotide exchange factor SOS. This interaction is mediated by the SH3 domains of NCK. These findings suggest that NCK can regulate p21ras-dependent gene transcription through interaction with SOS protein.