Quantitative expression of thrombopoietin receptor on leukaemia cells from patients with acute myeloid leukaemia and acute lymphoblastic leukaemia
Quantitative expression of thrombopoietin receptor on leukaemia cells from patients with acute myeloid leukaemia and acute lymphoblastic leukaemia
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急性髓细胞白血病和急性淋巴细胞白血病患者白血病细胞血小板生成素受体的定量表达
DOI：
10.1046/j.1365-2141.1998.00558.x
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发表时间：
1998
影响因子：
6.5
通讯作者：
R. Ohno
中科院分区：
文献类型：
--
作者：
A. Takeshita;K. Shinjo;M. Izumi;P. Ling;S. Nakamura;K. Naito;K. Ohnishi;R. Ohno
使用非同位素配体结合试验，我们定量检测了128例急性髓性白血病（AML）或急性淋巴细胞白血病（ALL）患者白血病细胞上人血小板生成素（TPO）受体（TPO‐R）的量。TPO‐R在114例AML病例中的53例（47%）中表达，TPO体外治疗导致66例AML病例中的13例（20%）白血病细胞增殖（刺激指数>1.5）。TPO水平与FAB分类无关，FAB‐M7 AML除外。所有5例FAB‐M7病例均表达TPO‐R，并且检查的3例FAB‐M7中有1例显示对TPO的体外增殖反应。尽管TPO‐R的量与增殖反应之间没有显著相关性（r=0.3125），但所有显示体外反应的AML病例都有TPO‐R表达。 TPO‐R量与CD表型或粒细胞集落刺激因子（G-CSF）受体量之间无相关性。TPO-R也在14例ALL中的2例（14%）中表达，只有这2例对TPO有体外增殖反应。一个只有淋巴样抗原，另一个同时有淋巴样和髓样抗原。我们的研究结果表明，一些白血病细胞表达功能活性的TPO‐R。
Using a non‐isotopic ligand binding assay, we quantitatively examined the amount of human thrombopoietin (TPO) receptor (TPO‐R) on leukaemia cells from 128 patients with acute myeloid leukaemia (AML) or acute lymphoblastic leukaemia (ALL). The TPO‐R was expressed in 53 (47%) of 114 AML cases, and an in vitro treatment with TPO led to proliferation (stimulation index >1.5) of leukaemia cells in 13 (20%) of 66 AML cases examined. The TPO levels had no relation to the FAB classification except for FAB‐M7 AML. All five FAB‐M7 cases expressed TPO‐R, and one of three FAB‐M7 examined showed in vitro proliferative response to TPO. Although there was no significant correlation (r = 0.3125) between the amount of TPO‐R and the proliferative response, all of the AML cases which showed the in vitro response had TPO‐R expression. There was no relationship between TPO‐R amount and CD phenotypes, or the amount of granulocyte‐colony stimulating factor (G‐CSF) receptor. TPO‐R was also expressed in two (14%) of 14 cases of ALL, and only these two cases had in vitro proliferative response to TPO. One had only lymphoid antigens, and the other had both lymphoid and myeloid antigens. Our results suggest that some leukaemia cells express functionally active TPO‐R.
影响因子：
2.2
作者：
BUTTKE, TM;MCCUBREY, JA;OWEN, TC
通讯作者：
OWEN, TC
人类胎儿发育过程中 CD34、CD38- 骨髓祖细胞生长特性的变化。
影响因子：
20.3
作者：
Waller,EK;Huang,S;Terstappen,L
通讯作者：
Terstappen,L