REPLICATION AND PLAQUE ASSAY OF INFLUENZA VIRUS IN AN ESTABLISHED LINE OF CANINE KIDNEY CELLS
REPLICATION AND PLAQUE ASSAY OF INFLUENZA VIRUS IN AN ESTABLISHED LINE OF CANINE KIDNEY CELLS
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DOI：
10.1128/aem.16.4.588-594.1968
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发表时间：
1968-01-01
期刊：
影响因子：
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通讯作者：
SMITH, TF
中科院分区：
文献类型：
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作者：
GAUSH, CR;SMITH, TF
在已建立的犬肾细胞系（MDCK-USD）中开发了A型和B型流感病毒的空斑检测系统。除了均匀的易感细胞外，一致的空斑产生取决于使用高度纯化的琼脂（琼脂糖）。该定量系统用于测定流感病毒的吸附、合成和热灭活速率，以及测定剂量响应曲线。MDCK-USD系和亲本MDCK系的空斑试验表明，后者对A/Swine和A2/Japan 305病毒更敏感。含胚鸡卵和MDCK-USD中标准病毒合并液的滴定表明，细胞培养系统与卵内试验一样灵敏。
A plaque assay system was developed for types A and B influenza viruses in an established line of canine kidney cells (MDCK-USD). In addition to a homogeneous susceptible cell, consistent plaque production depends on the use of highly purified agar (Agarose). This quantitative system was used to determine the rate of adsorption, synthesis, and thermal inactivation of influenza viruses, as well as to determine a dose response curve. Plaque assays on the MDCK-USD line and the parent MDCK line showed that the latter was more sensitive to A/Swine and A2/Japan 305 viruses. Titration of standard virus pools in embryonated eggs and MDCK-USD indicated that the cell culture system was as sensitive as the in ovo assay.