A lateral flow immunoassay for straightforward determination of fumonisin mycotoxins based on the quenching of the fluorescence of CdSe/ZnS quantum dots by gold and silver nanoparticles
A lateral flow immunoassay for straightforward determination of fumonisin mycotoxins based on the quenching of the fluorescence of CdSe/ZnS quantum dots by gold and silver nanoparticles
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DOI：
10.1007/s00604-017-2642-0
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发表时间：
2018-02-01
影响因子：
5.7
通讯作者：
Baggiani, Claudio
中科院分区：
文献类型：
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作者：
Anfossi, Laura;Di Nardo, Fabio;Baggiani, Claudio
建立了一种检测伏马菌素真菌毒素的横向流动免疫分析法（LFIA）。在激发/发射波长为365/525 nm的CdSe/ZnS量子点（QDs）中，银纳米粒子（SNPs）或金纳米粒子（GNPs）的加入抑制了量子点（QDs）的荧光，因为SNPs重叠了量子点的激发能带，GNPs重叠了量子点的发射能带。添加伏马菌素后，量子点的荧光恢复，通过监测量子点荧光强度的变化，可以在“阳性模式”下对目标霉菌毒素进行敏感检测。SNPs被发现是最有效的猝灭剂，而GNPs的使用可以有效地恢复荧光，并且可以在LFIA中使用。该方法成功地应用于加标玉米粉样品中伏马菌素的荧光测定。目视检测限为ng。毫升(1)水平。这比基于使用传统金NPs的比色LFIA低四倍。
A lateral flow immunoassay (LFIA) was developed for the determination of fumonisin mycotoxins. The fluorescence of CdSe/ZnS quantum dots (QDs), observed at excitation/emission wavelengths of 365/525 nm, is suppressed by the addition of silver nanoparticles (SNPs) or gold nanoparticles (GNPs) because SNPs overlap the excitation bands of the QDs, and GNPs overlap the emission bands. The fluorescence of the QDs is recovered upon addition of fumonisins, allowing for the sensitive detection in "positive mode" of the target mycotoxin by monitoring the changes of the QDs fluorescence intensity. The SNPs are found to be the most effective quenchers, while the use of GNPs allows for an efficient recovery of fluorescence and can be employed in the LFIA. The method was successfully applied to the fluorometric determination of fumonisins in spiked maize flour samples. The visual detection limit is at the ng.mL(-1) level. This is four times lower compared to the colorimetric LFIA based on the use of the conventional gold NPs.