Identification and expression pattern analysis of the glucosinolate biosynthetic gene BoCYP83B1 from broccoli
Identification and expression pattern analysis of the glucosinolate biosynthetic gene BoCYP83B1 from broccoli
复制标题
DOI：
10.1007/s10535-018-0797-0
复制
发表时间：
2018-09
影响因子：
1.5
通讯作者：
XU R.;W. Kong;Y. Peng;K. Zhang;L. R.;L. J
中科院分区：
文献类型：
--
作者：
XU R.;W. Kong;Y. Peng;K. Zhang;L. R.;L. J
硫代葡萄糖苷是一个分支的氨基酸衍生的代谢产物，这是专门发现在nanocales。在拟南芥中，色氨酸衍生的吲哚硫代葡萄糖苷具有很强的抗菌活性和潜在的信号传导功能，是植物防御多种病原体和食草动物所必需的。CYP 83 B1是吲哚硫苷生物合成途径中的一个重要酶，它氧化吲哚-3-乙酸（IAA）的前体吲哚-3-乙醛肟。本研究报道了甘蓝中aCYP 83 B1基因的分离和表达特征。var.italicaPlenck，我们将其命名为BoCYP 83 B1。BoCYP 83 B1在拟南芥中的过表达导致硫代葡萄糖苷谱和早花表型的改变。通过在拟南芥中表达BoCYP 83 B1启动子调控下的报告基因β-葡萄糖醛酸酶，分析了BoCYP 83 B1在正常生长条件下以及在几种激素和胁迫下的空间表达模式。BoCYP 83 B1主要在整个植株的维管组织中表达。茉莉酸甲酯、1-氨基-1-环丙烷羧酸、水杨酸、赤霉素和IAA都能强烈诱导该基因的表达，表明它参与了复杂的信号转导途径。甘露醇、NaCl、UV和Flagelin 22显著上调BoCYP 83 B1的表达，表明其可能在胁迫反应中起作用。有趣的是，BoCYP 83 B1对SA和NaCl的反应具有组织特异性。因此，BoCYP 83 B1在不同组织中可能具有不同的功能。
Glucosinolates are a branch of amino acid-derived metabolites, which are specifically found inBrassicales. InArabidopsis, tryptophan derived indolic glucosinolates are required for plant defense against a wide range of pathogens and herbivores due to their strong antimicrobial activity and potential signaling function. An important enzyme in indolic glucosinolate biosynthesis pathway isCYP83B1, which oxidizes indole-3-acetaldoxime, a precursor of indole-3-acetic acid (IAA). In this study, we reported isolation and expression characterization of aCYP83B1gene fromBrassica oleraceaL. var.italicaPlenck, which we termedBoCYP83B1. Overexpression ofBoCYP83B1inArabidopsisresulted in an altered glucosinolate profile and early flowering phenotype. By expressing the reporter gene β-glucuronidase under the control of theBoCYP83B1promoter in Arabidopsis, we analyzed the spatial expression pattern ofBoCYP83B1under normal growth conditions as well as in response to several hormones and stresses. TheBoCYP83B1was primarily expressed in vascular tissue through the almost whole plant. It was strongly induced by methyl jasmonate, 1-amino-1-cyclopropanecarboxylic acid, salicylic acid (SA), gibberellin, and IAA, suggesting its involvement in complex signaling pathways. Mannitol, NaCl, UV, and Flagelin 22 significantly up-regulatedBoCYP83B1expression, indicating its possible role in stress response. Interestingly, the response ofBoCYP83B1to SA and NaCl showed tissue specificity. Thus,BoCYP83B1might have different functions in different tissues.