IN-VIVO MEASUREMENT OF OXYGEN CONCENTRATION USING SONOCHEMICALLY SYNTHESIZED MICROSPHERES
IN-VIVO MEASUREMENT OF OXYGEN CONCENTRATION USING SONOCHEMICALLY SYNTHESIZED MICROSPHERES
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DOI：
10.1016/s0006-3495(94)80551-x
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发表时间：
1994-08-01
影响因子：
3.4
通讯作者：
WANG, W
中科院分区：
文献类型：
--
作者：
LIU, KJ;GRINSTAFF, MW;WANG, W
首次使用高强度超声合成了填充有溶解在有机液体中的氮氧化合物的蛋白质微球;这些微球用于测量活生物系统中的氧浓度。微球的平均尺寸为2.5 μ m，蛋白质外壳可渗透氧气。由于氧在有机溶剂中的较高溶解度，将氮氧化合物封装到微球中大大增加了电子顺磁共振信号线宽度对氧的灵敏度。包封还保护氮氧化物免于生物还原。将微球静脉注射到小鼠体内后70分钟内，未观察到电子顺磁共振信号强度降低。利用该微球，通过限制血流、麻醉和呼吸气体中氧含量的变化来测量体内氧浓度的变化。
Proteinaceous microspheres filled with nitroxides dissolved in an organic liquid have been synthesized for the first time using high intensity ultrasound; these were used to measure oxygen concentrations in living biological systems. The microspheres have an average size of 2.5 mu m, and the proteinaceous shell is permeable to oxygen. Encapsulation of the nitroxides into the microsphere greatly increased the sensitivity of the electron paramagnetic resonance signal line width to oxygen because of the higher solubility of oxygen in organic solvents. The encapsulation also protected the nitroxide from bioreduction. No decrease in intensity of the electron paramagnetic resonance signal was observed during 70 min after intravenous injection of the microspheres into a mouse. Measurement of the changes in oxygen concentration in vivo by means of restriction of blood flow, anesthesia, and change of oxygen content in the respired gas were made using these microspheres.