Nondestructive micro-computed tomography for biological imaging and quantification of scaffold-bone interaction in vivo
Nondestructive micro-computed tomography for biological imaging and quantification of scaffold-bone interaction in vivo
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DOI：
10.1016/j.biomaterials.2007.01.017
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发表时间：
2007-05-01
期刊：
影响因子：
14
通讯作者：
Mueller, Ralph
中科院分区：
文献类型：
--
作者：
van Lenthe, G. Harry;Hagenmueller, Henri;Mueller, Ralph
支架，也称为生物支架，在所有组织工程应用中都需要作为细胞和生化因子的载体，作为提供适当机械条件的构建体，或作为两者的组合。本文的目的是提出最近的发展，在微计算机断层扫描（μ CT）分析的支架。重点将放在骨研究中的成像和定量方面，并将处理支架结构的评估及其如何与骨组织相互作用。我们表明，微结构成像是一种非破坏性和非侵入性的程序，允许精确的三维（3D）测量支架结构。直接基于mu CT的图像分析允许准确地量化支架孔隙率、表面积和3D测量，例如孔径、孔分布和支柱厚度;此外，它允许精确测量骨生长到支架中及其表面上。该方法可用于支架制造过程的质量控制，以评估支架降解动力学，并评估骨组织反应。更重要的是，结合生物反应器或体内动物模型，mu CT允许定性和定量评估支架中骨组织形成的空间和时间矿化;这种纵向研究改善了对支架结构引起的骨反应的评估。计算模型将有助于进一步分析这些数据，以提高我们对骨形成的机械和生化刺激的理解，并可能提供有价值的知识，以优化支架设计。(c)2007爱思唯尔有限公司版权所有。
Scaffolds, also called bioscaffolds, are needed in all tissue engineering applications as carriers for cells and biochemical factors, as constructs providing appropriate mechanical conditions, or as a combination of the two. The aim of this paper is to present recent developments in micro-computed tomography (mu CT) analyses of scaffolds. The focus will be on imaging and quantification aspects in bone research, and will deal with the assessment of scaffold architecture and how it interacts with bone tissue. We show that microarchitectural imaging is a nondestructive and noninvasive procedure that allows a precise three-dimensional (3D) measurement of scaffold architecture. Direct mu CT-based image analysis allows to accurately quantify scaffold porosity, surface area, and 3D measures such as pore size, pore distribution, and strut thickness; furthermore, it allows for a precise measurement of bone growth into the scaffold and onto its surface. This methodology is useful for quality control of scaffold fabrication processes, to assess scaffold degradation kinetics, and to assess bone tissue response. Even more so, in combination with bioreactors or in vivo animal models, mu CT allows to qualitatively and quantitatively assess the spatial and temporal mineralization of bone tissue formation in scaffolds; such longitudinal studies improve the assessment of bone response due to scaffold architecture. Computational models will be helpful in further analyses of these data in order to improve our understanding of mechanical and biochemical stimuli on bone formation, and are likely to provide valuable knowledge to optimize scaffold design. (c) 2007 Elsevier Ltd. All rights reserved.