Complete genome sequence of yam chlorotic necrosis virus, a novel macluravirus infecting yam
Complete genome sequence of yam chlorotic necrosis virus, a novel macluravirus infecting yam
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山药褪绿坏死病毒（一种感染山药的新型Macluravirus）的完整基因组序列
DOI：
10.1007/s00705-018-3851-5
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发表时间：
2018-08-01
影响因子：
2.7
通讯作者：
Li, Fan
中科院分区：
文献类型：
--
作者：
Lan, Pingxiu;Meng, Yu;Li, Fan
从我国具有退绿和坏死症状的山药植株中测定了一种新的斑点病毒属成员的全基因组序列。该基因组RNA由8，261个核苷酸（nt）组成，不包括3 '-末端poly（A）尾，含有一个长的开放阅读框（ORF），编码2，627个氨基酸的大的推定多聚蛋白。它的基因组结构是典型的macluraviruses，它缺乏P1蛋白，N-末端HC-Pro，和D-A-G基序蚜虫传播中发现的马铃薯Y病毒。该病毒与Macluravirus属的其他成员在基因组序列水平上具有56.3-63.8%的序列同一性，在多蛋白序列水平上具有49.7-63.9%的序列同一性。基于马铃薯Y病毒科代表性成员的完整多蛋白序列的系统发育分析清楚地将该病毒置于Macluravirus属内。这些结果表明，该病毒，暂命名为“山药褪绿坏死病毒”（YCNV），应被认为是一个新的物种在Macluravirus属的成员。
The complete genome sequence of a novel member of the genus Macluravirus was determined from yam plants with chlorotic and necrotic symptoms in China. The genomic RNA consists of 8,261 nucleotides (nt) excluding the 3'-terminal poly(A) tail, containing one long open reading frame (ORF) encoding a large putative polyprotein of 2,627 amino acids. Its genomic structure is typical of macluraviruses, which lack the P1 protein, N-terminal HC-Pro, and D-A-G motif for aphid transmission that are found in potyviruses. The virus shares 56.3-63.8% sequence identity at the genome sequence level and 49.7-63.9% at the polyprotein sequence level with other members of the genus Macluravirus. Phylogenetic analysis based on the complete polyprotein sequence of representative members of the family Potyviridae clearly places the virus within the genus Macluravirus. These results suggest that the virus, tentatively named "yam chlorotic necrosis virus" (YCNV), should be considered a member of a novel species in the genus Macluravirus.