Titanium nanotopography induces osteocyte lacunar-canalicular networks to strengthen osseointegration
Titanium nanotopography induces osteocyte lacunar-canalicular networks to strengthen osseointegration
复制标题
DOI：
10.1016/j.actbio.2022.08.023
复制
发表时间：
2022-09-29
影响因子：
9.7
通讯作者：
Egusa, Hiroshi
中科院分区：
文献类型：
--
作者：
He, Xindie;Yamada, Masahiro;Egusa, Hiroshi
骨细胞网络结构与骨转换密切相关。细胞机械感应系统通过增强粘着斑调节骨细胞树突的形成。因此，钛表面纳米形貌可能会影响骨细胞网络结构，改善种植体周围骨组织质量，从而加强骨锚定种植体的骨整合。我们的目的是研究钛纳米表面对骨细胞腔隙-小管网络发育和牙种植体骨整合的影响。碱性蚀刻产生了具有各向异性图案化的致密纳米尖峰、超亲水性和羟基的钛纳米表面。在钛纳米表面上培养的MLO-Y 4小鼠骨细胞样细胞产生了神经元样树突，具有增加的粘着斑和间隙连接。与在机械加工或酸蚀刻的微粗糙钛表面上培养的细胞相比，在钛纳米表面上培养的骨细胞的成熟得到促进。在纳米粗糙钛表面上，在I型三维胶原凝胶中培养7天的骨细胞显示出与高度发达的树突和间隙连接的良好互连性，而在其他钛表面上观察到的互连性较差。即使保持超亲水性和羟基，各向异性图案化的纳米钉的损失降低了碱性蚀刻的钛纳米表面上培养的骨细胞中间隙连接的表达。将钛纳米表面种植体植入野生型大鼠上颌骨四周后，发现具有大量树突的骨细胞直接附着在种植体表面，在纳米粗糙钛种植体周围形成发育良好的腔隙-小管网络。纳米粗糙钛种植体的骨结合强度明显高于微粗糙钛种植体。这些数据表明，钛纳米表面促进骨细胞腔隙-小管网络的发展，通过nan-otopographical线索和加强骨整合。
Osteocyte network architecture is closely associated with bone turnover. The cellular mechanosensing system regulates osteocyte dendrite formation by enhancing focal adhesion. Therefore, titanium surface nanotopography might affect osteocyte network architecture and improve the peri-implant bone tissue quality, leading to strengthened osseointegration of bone-anchored implants. We aimed to investigate the effects of titanium nanosurfaces on the development of osteocyte lacunar-canalicular networks and osseointegration of dental implants. Alkaline etching created titanium nanosurfaces with anisotropically patterned dense nanospikes, superhydrophilicity, and hydroxyl groups. MLO-Y4 mouse osteocyte-like cells cultured on titanium nanosurfaces developed neuron-like dendrites with increased focal adhesion as-sembly and gap junctions. Maturation was promoted in osteocytes cultured on titanium nanosurfaces compared to cells cultured on machined or acid-etched micro-roughened titanium surfaces. Osteocytes cultured in type I three-dimensional collagen gels for seven days on nano-roughened titanium surfaces displayed well-developed interconnectivity with highly developed dendrites and gap junctions compared to the poor interconnectivity observed on the other titanium surfaces. Even if superhydrophilicity and hydroxyl groups were maintained, the loss of anisotropy-patterned nanospikes reduced expression of gap junction in osteocytes cultured on alkaline-etched titanium nanosurfaces. Four weeks after placing the ti-tanium nanosurface implants in the upper jawbone of wild-type rats, osteocytes with numerous dendrites were found directly attached to the implant surface, forming well-developed lacunar-canalicular networks around the nano-roughened titanium implants. The osseointegration strength of the nano-roughened titanium implants was significantly higher than that of the micro-roughened titanium implants. These data indicate that titanium nanosurfaces promote osteocyte lacunar-canalicular network development via nan-otopographical cues and strengthen osseointegration.