Galectin-7, a marker of all types of stratified epithelia
Galectin-7, a marker of all types of stratified epithelia
复制标题
DOI：
10.1046/j.1432-0436.1998.6330159.x
复制
发表时间：
1998-07-01
期刊：
影响因子：
2.9
通讯作者：
Darmon, M
中科院分区：
文献类型：
--
作者：
Magnaldo, T;Fowlis, D;Darmon, M
Galectin-7是我们先前在人类中克隆的凝集素家族的14-kDA成员。它的表达被发现在所有阶段的分化的人表皮和减少，但不抑制口腔上皮化生重建时，由维甲酸诱导。这表明半乳凝素-7可能是角化和非角化复层上皮的标志物。为了证实这一假设，我们克隆了大鼠和小鼠的cDNA，并产生了针对合成肽的特异性抗血清。通过原位杂交和免疫标记研究了各种人、大鼠和小鼠上皮细胞的半乳糖凝集素-7 mRNA和蛋白的分布。Galectin-7在毛囊间表皮和毛囊外根鞘中表达，但在毛基质和皮脂腺中均不表达。食管上皮、口腔上皮、角膜、胸腺的Hassal小体均有表达，单纯上皮和移行上皮无表达。因此，半乳糖凝集素-7可以被认为是角质形成细胞的所有亚型的标志物。在这方面，它既不同于“基底特异性”角蛋白K5-K14，也不同于“基底上特异性”标记物，如角蛋白K1-K10和外皮蛋白。半乳糖凝集素-7也不同于一些桥粒蛋白，其存在于所有类型的上皮细胞和心肌中。半乳糖凝集素-7在培养的癌细胞系中不存在，并且在人癌和用两阶段致癌方案产生的鼠肿瘤中均减少。
Galectin-7 is a 14-kDA member of the lectin family we have previously cloned in the human. Its expression was found at all stages of differentiation of the human epidermis and was reduced but not suppressed when oral metaplasia of reconstructed epidermis was induced by retinoic acid. This suggested that galectin-7 could be a marker of both keratinized and non-keratinized stratified epithelia. To ascertain this hypothesis, we cloned the rat and the mouse cDNAs and produced a specific antiserum raised against a synthetic peptide. The distribution of galectin-7 mRNAs and protein was studied by in situ hybridization and immunolabelling of various human, rat and mouse epithelia. Galectin-7 was found to be expressed in interfollicular epidermis and in the outer root sheath of the hair follicle, but not in the hair matrix, nor in the sebaceous glands. It was present in esophagus and oral epithelia, cornea, Hassal's corpuscles of the thymus, but not in simple and transitional epithelia. Galectin-7 can thus be considered as a marker of all subtypes of keratinocytes. In that respect it differs from both "basal-specific" keratins K5-K14 and from "suprabasal-specific" markers such as keratins K1-K10 and involucrin. Galectin-7 also differs from some desmosomal proteins, which are present in all types of epithelia and in myocardium. Galectin-7 was absent from cultured carcinoma cell lines and was reduced both in human carcinomas and in murine tumors produced with the two-stage carcinogenesis protocol.