Heat-shock proteins of Drosophila are associated with nuclease-resistant, high-salt-resistant nuclear structures.
Heat-shock proteins of Drosophila are associated with nuclease-resistant, high-salt-resistant nuclear structures.
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DOI：
10.1083/jcb.90.3.793
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发表时间：
1981-09
影响因子：
7.8
通讯作者：
Varshavsky, A
中科院分区：
文献类型：
--
作者：
Levinger, L;Varshavsky, A
最近的放射自显影显示，果蝇培养细胞在热休克反应（HSPs）过程中产生的蛋白质在很大程度上局限于细胞核。我们在这里报告说，核热休克蛋白与核小体溶解处理葡萄球菌核酸酶在低离子强度，也没有热休克蛋白释放提取高盐，溶解了大部分剩余的组蛋白和DNA。核热休克蛋白的可能功能进行了讨论。
Proteins produced in cultured Drosophila cells during the heat-shock response (HSPs) were recently shown by autoradiography to be confined in large measure to the cell nucleus. We report here that nuclear HSPs are not associated with nucleosomes solubilizes by treatment with staphylococcal nuclease at low ionic strength nor are HSPs released by extraction with high salt, which solubilized most of the remaining histones and DNA. Possible functions of nuclear HSPs are discussed.