Plasma direct injection high-performance liquid chromatographic method for simultaneously determining E3810 enantiomers and their metabolites by using flavoprotein-conjugated column.
Plasma direct injection high-performance liquid chromatographic method for simultaneously determining E3810 enantiomers and their metabolites by using flavoprotein-conjugated column.
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DOI：
10.1021/js960172n
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发表时间：
1996-09
影响因子：
3.8
通讯作者：
N. Mano;Y. Oda;S. Takakuwa;S. Chiku;H. Nakata;N. Asakawa
中科院分区：
文献类型：
--
作者：
N. Mano;Y. Oda;S. Takakuwa;S. Chiku;H. Nakata;N. Asakawa
采用柱切换技术建立了同时测定E3810及其代谢物(M1-M4)的高效液相色谱方法。亲和素色谱柱用于在线前处理，以排除血浆蛋白，允许直接注射大量血浆。黄素蛋白偶联手性固定相在梯度洗脱模式下对E3810和4种代谢物进行基线分离。还分离了E3810和M3的对映体。该方法简便、快速、准确、精密度高。由于不使用提取程序，可能涉及不同代谢物的不同回收率，因此不需要内标。方法用于Beagle犬静脉注射E3810后血浆中E3810及其代谢产物的分析。
A high-performance liquid chromatographic method using the column-switching technique was developed for the simultaneous determination of E3810 and its metabolites (M1-M4). An avidin column was used for in-line pretreatment to exclude plasma proteins, allowing direct injection of a large volume of plasma. A flavoprotein-conjugated chiral stationary phase in a gradient elution mode then gave baseline separation of E3810 and the four metabolites. The enantiomers of E3810 and M3 were also separated. The method is simple, rapid, accurate, and precise. Since no extraction procedure is employed, which might involve different recoveries of different metabolites, no internal standard is necessary. The method was applied to analyze E3810 and its metabolites in plasma after intravenous injection of the drug in beagle dogs.