REST/NRSF governs the expression of dense-core vesicle gliosecretion in astrocytes.
REST/NRSF governs the expression of dense-core vesicle gliosecretion in astrocytes.
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DOI：
10.1083/jcb.201010126
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发表时间：
2011-05-02
期刊：
影响因子：
--
通讯作者：
Meldolesi J
中科院分区：
文献类型：
--
作者：
Prada I;Marchaland J;Podini P;Magrassi L;D'Alessandro R;Bezzi P;Meldolesi J
The REST/NRSF transcriptional repressor prevents cultured astrocytes from forming DCVs, and its variable expression in human brain cortex astrocytes may account for their functional heterogeneity. Astrocytes are the brain nonnerve cells that are competent for gliosecretion, i.e., for expression and regulated exocytosis of clear and dense-core vesicles (DCVs). We investigated whether expression of astrocyte DCVs is governed by RE-1–silencing transcription factor (REST)/neuron-restrictive silencer factor (NRSF), the transcription repressor that orchestrates nerve cell differentiation. Rat astrocyte cultures exhibited high levels of REST and expressed neither DCVs nor their markers (granins, peptides, and membrane proteins). Transfection of a dominant-negative construct of REST induced the appearance of DCVs filled with secretogranin 2 and neuropeptide Y (NPY) and distinct from other organelles. Total internal reflection fluorescence analysis revealed NPY–monomeric red fluorescent protein–labeled DCVs to undergo Ca2+-dependent exocytosis, which was largely prevented by botulinum toxin B. In the I–II layers of the human temporal brain cortex, all neurons and microglia exhibited the expected inappreciable and high levels of REST, respectively. In contrast, astrocyte REST was variable, going from inappreciable to high, and accompanied by a variable expression of DCVs. In conclusion, astrocyte DCV expression and gliosecretion are governed by REST. The variable in situ REST levels may contribute to the well-known structural/functional heterogeneity of astrocytes.
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影响因子：
3.7
作者：
Abrajano JJ;Qureshi IA;Gokhan S;Zheng D;Bergman A;Mehler MF
通讯作者：
Mehler MF
影响因子：
4.8
作者：
Ekici, Myriam;Hohl, Mathias;Thiel, Gerald
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Thiel, Gerald
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4.8
作者：
Coco, S;Calegari, F;Verderio, C
通讯作者：
Verderio, C
影响因子：
4.8
作者：
Calegari, F;Coco, S;Rosa, P
通讯作者：
Rosa, P
DOI：
10.1073/pnas.0401827101
发表时间：
2004-07-13
影响因子：
11.1
作者：
Bruce, AW;Donaldson, IJ;Buckley, NJ
通讯作者：
Buckley, NJ