Nerve growth factor enhances survival and cytotoxic activity of human eosinophils
Nerve growth factor enhances survival and cytotoxic activity of human eosinophils
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DOI：
10.1046/j.1365-2141.1996.5151055.x
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发表时间：
1996-05-01
影响因子：
6.5
通讯作者：
Matsuda, H
中科院分区：
文献类型：
--
作者：
Hamada, A;Watanabe, N;Matsuda, H
血型嗜酸性粒细胞向组织型嗜酸性粒细胞的表型变化是由多种细胞因子诱导的。我们研究了神经生长因子（NGF）作为一种候选组成细胞因子的作用，它能够诱导嗜酸性粒细胞的表型变化，通过添加NGF (50 ng/ml)，培养4 d的人外周血嗜酸性粒细胞的存活率从平均26%提高到平均50% （P < 0.001）。添加NGF （50 ng/ml）后，嗜酸性粒细胞的细胞毒活性提高了2 ~ 3倍（P < 0.05），并通过盲孔室法证实了NGF的嗜酸性粒细胞趋化活性。由于NGF是由局部组织中的多种细胞组成的，因此提示NGF可能是在没有免疫刺激的情况下导致组织型嗜酸性粒细胞表型变化的细胞因子。
Phenotypic change of blood-type eosinophils to tissue-type eosinophils is induced by various cytokines. We examined the effect of nerve growth factor (NGF) as a candidate for a constitutive cytokine which is able to induce the phenotypic change of eosinophils, The viability of human peripheral blood eosinophils cultured for 4 d was increased from a mean of 26% to a mean of 50% (P < 0.001) by the addition of NGF (50 ng/ml). Cytotoxic activity of eosinophils determined by larvicidal activity was increased 2-3-fold by the addition of NGF (50 ng/ml) (P < 0.05), Furthermore, eosinophil chemotactic activity of NGF was demonstrated by the blind well chamber method. Since NGF is produced constitutively from various kinds of cells in local tissues, it is suggested that NGF might be a cytokine responsible for phenotypic change to tissue type eosinophils in conditions without immune stimuli.