SUCROSE MIMICS THE LIGHT INDUCTION OF ARABIDOPSIS NITRATE REDUCTASE GENE-TRANSCRIPTION
SUCROSE MIMICS THE LIGHT INDUCTION OF ARABIDOPSIS NITRATE REDUCTASE GENE-TRANSCRIPTION
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DOI：
10.1073/pnas.89.5.1861
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发表时间：
1992-03-01
影响因子：
11.1
通讯作者：
CONKLING, MA
中科院分区：
文献类型：
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作者：
CHENG, CL;ACEDO, GN;CONKLING, MA
硝酸还原酶是硝酸盐同化过程中的第一个酶，处于硝酸盐同化和碳固定两条耗能途径的交叉点。光调节许多高等植物碳固定基因的表达，也调节硝酸还原酶基因的表达。硝酸还原酶位于细胞质中，其还原剂不是来自光合作用，而是来自碳水化合物催化。这种关系促使我们研究光通过光合作用在硝酸还原酶基因表达调控中可能发挥的间接作用。我们表明，蔗糖可以取代光诱导增加硝酸还原酶mRNA积累在黑暗适应的绿色拟南芥植物。我们进一步表明，蔗糖单独是足够的硝酸还原酶基因在黄化的拟南芥植物的充分表达。最后，使用一个报告基因，我们表明，2.7-脱氢酶区域的5'侧翼序列的硝酸还原酶基因是足以赋予光或蔗糖的反应。
Nitrate reductase, the first enzyme in nitrate assimilation, is located at the crossroad of two energy-consuming pathways: nitrate assimilation and carbon fixation. Light, which regulates the expression of many higher-plant carbon fixation genes, also regulates nitrate reductase gene expression. Located in the cytosol, nitrate reductase obtains its reductant not from photosynthesis but from carbohydrate catabolism. This relationship prompted us to investigate the indirect role that light might play, via photosynthesis, in the regulation of nitrate reductase gene expression. We show that sucrose can replace light in eliciting an increase of nitrate reductase mRNA accumulation in dark-adapted green Arabidopsis plants. We show further that sucrose alone is sufficient for the full expression of nitrate reductase genes in etiolated Arabidopsis plants. Finally, using a reporter gene, we show that a 2.7-kilobase region of 5' flanking sequence of the nitrate reductase gene is sufficient to confer the light or the sucrose response.