Coordinate induction of electrophoretic variants of juvenile hormone esterase.
Coordinate induction of electrophoretic variants of juvenile hormone esterase.
复制标题
保幼激素酯酶电泳变体的协调诱导。
DOI：
10.1016/0006-291x(89)90827-9
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发表时间：
1989
影响因子：
3.1
通讯作者：
Manczak,M
中科院分区：
文献类型：
--
作者：
Jones,G;Manczak,M
在粉纹夜蛾（Trichoplusia ni）末龄幼虫变态定型前后的血淋巴中发现保幼激素酯酶（JHE）的主要和次要电泳变异体。两种主要形式的活动的平均比率在活动的最后两个体育场高峰期是相似的。免疫学分析表明，血淋巴中的JHE浓度在此期间的酶活性水平，并没有推定的高分子量，无活性的形式被检测到。免疫学分析提供了第一个证据的相关性的主要和次要形式。激素刺激后，两种主要形式的浓度同时增加，并以相似的比例，这表明电荷变化，至少对于这两种主要形式，是不是一个点的激素或发育调节JHE。
Major and minor electrophoretic variants of juvenile hormone esterase (JHE) were found in the hemolymph of last instar larvae of Trichoplusia ni, both before and after metamorphic commitment. The average ratios of activity of the two major forms were similar during both last stadium peaks in activity. Immunological analysis showed that the hemolymph concentration of JHE during this stadium paralleled the level of enzymatic activity, and no putative higher molecular weight, inactive forms were detected. Immunological analysis provided the first evidence of relatedness of major and minor forms. After hormonal stimulation, the concentration of the two major forms increased concomitantly and by a similar proportion, suggesting that charge variation, at least for these two major forms, is not a point of hormonal or developmental regulation of JHE.