Fluorescent in situ hybridization on isolated tumor cell nuclei:: A sensitive method for 1p and 19q deletion analysis in paraffin-embedded oligodendroglial tumor specimens
Fluorescent in situ hybridization on isolated tumor cell nuclei:: A sensitive method for 1p and 19q deletion analysis in paraffin-embedded oligodendroglial tumor specimens
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DOI：
10.1097/01.mp.0000076981.90281.bf
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发表时间：
2003-07-01
期刊：
影响因子：
7.5
通讯作者：
Hainfellner, JA
中科院分区：
文献类型：
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作者：
Gelpi, E;Ambros, IM;Hainfellner, JA
在少突胶质细胞肿瘤中，1p 和 19q 染色体物质的丢失与化疗敏感性和延长生存期相关。因此，1p/19q 检测越来越多地被提议用于脑肿瘤诊断和预后评估。荧光原位杂交 (FISH) 是研究石蜡包埋组织中 1p/19q 状态的经典技术。该方法的一个主要限制是肿瘤细胞核的截断，使杂交结果的评估变得复杂。在我们的研究中，我们使用 FISH 对制备为细胞离心涂片制剂的分离的整个肿瘤细胞核进行 FISH，分析了一系列 79 例少突胶质细胞瘤（49 例少突胶质细胞瘤，30 例少突星形细胞瘤，WHO：57 例 11 级、22 例 III 级肿瘤）和对照（神经胶质脑组织：n = 4，弥漫性低级别星形细胞瘤：n = 4）的 1p 和 19q 状态，从而绕过了核截断问题。为了解释 FISH 结果，我们使用 SIOP-欧洲神经母细胞瘤研究组定义的共识标准来分析周围神经母细胞肿瘤。 FISH 的 1p 和 19q 的可解释结果分别为 98.7% 和 92.1%。染色体 1p/19q 改变包括缺失（1p：79.5%，19q：80%）和不平衡（1p：11.5%，19q：12.9%）。少突胶质细胞瘤中的 1p 畸变比少突星形细胞瘤中更常见（100% vs 75.9%，P = .001）。 1p/19q 改变的频率在 WHO 11 级或 III 级肿瘤或原发性和复发性肿瘤中没有显着差异。我们得出结论，应用 SIOP Europe 神经母细胞瘤对分离的细胞核进行 FISH。共识标准，是检测和解释少突胶质细胞肿瘤石蜡包埋组织标本中 1p 和 19q 畸变的灵敏方法。
In oligodendroglial neoplasms, losses of chromosomal material at 1p and 19q associate with chemosensitivity and prolonged survival. Thus, 1p/19q testing is increasingly proposed for use in brain tumor diagnosis and prognostic assessment. Fluorescent in situ hybridization (FISH) is a classic technique for investigation of 1p/19q status in paraffin-embedded tissues. A major limitation of this method is truncation of tumor cell nuclei complicating assessment of hybridization results. In our study, we analyzed 1p and 19q status in a series of 79 oligodendroglial neoplasms (49 oligodendrogliomas, 30 oligoastrocytomas, WHO: 57 Grade 11, 22 Grade III tumors) and controls (gliotic brain tissue: n = 4, diffuse low-grade astrocytoma: n = 4) using FISH on isolated whole tumor cell nuclei, prepared as cytospin preparations, thus bypassing the problem of nuclear truncation. For interpretation of FISH results, we used consensus criteria as defined by the SIOP-Europe Neuroblastoma Study Group for analysis of peripheral neuroblastic tumors. FISH yielded interpretable results in 98.7% for 1p and 92.1% for 19q. Chromosome 1p/19q alterations comprised deletions (1p: 79.5%, 19q: 80%) and imbalances (1p: 11.5%, 19q: 12.9%). 1p aberrations were more frequent in oligodendroglioma than in oligoastrocytoma (100% versus 75.9%, P = .001). The frequency of 1p/19q alterations was not significantly different in WHO Grade 11 or Grade III tumors or in primary and recurrent tumors. We conclude that FISH on isolated cell nuclei, with application of the SIOP Europe Neuroblastoma. consensus criteria, is a sensitive method for detection and interpretation of 1p and 19q aberrations in paraffin-embedded tissue specimens of oligodendroglial neoplasms.