Inhibitory avoidance learning altered ensemble activity of amygdaloid neurons in rats
Inhibitory avoidance learning altered ensemble activity of amygdaloid neurons in rats
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DOI：
10.1111/j.1460-9568.2004.03821.x
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发表时间：
2005-01-01
影响因子：
3.4
通讯作者：
Yen, CT
中科院分区：
文献类型：
--
作者：
Chang, CH;Liang, KC;Yen, CT
在这项研究中，我们研究了单个单位活动的杏仁核之前和之后，大鼠已获得了抑制性回避任务。Long-Evans大鼠在杏仁核中央核（CeA）或基底外侧复合体（BLC）长期植入微丝，使其适应避暗回避任务装置，共3次。在第四届会议上，实验组的大鼠收到了不可避免的footshock（3毫安，1秒），因为他们从明亮的一面到黑暗的一面的任务装置，而对照组中的大鼠收到了相同数量的冲击不同的设备。所有大鼠在电击训练后1天在任务装置中测试保持力。实验组大鼠比对照组大鼠表现出更好的保持力，因为它们在亮侧停留的时间更长。在驯化和测试期间，从留置的钢丝束记录杏仁核中的包围单位活动。从分离良好的杏仁核单位收集的数据表明，神经元放电习惯从第一到第三驯化会议。在测试过程中，实验组（而不是对照组）显示位于大脑两侧的CeA或BLC神经元的放电率升高。这些发现提供了第一个证据，表明学习抑制性回避任务导致在保留测试过程中杏仁核神经元放电增加。
In this study, we examined single-unit activity in the amygdala before and after a rat had acquired an inhibitory avoidance task. Long-Evans rats with microwires chronically implanted into the central nucleus (CeA) or basolateral complex (BLC) of the amygdala were acclimatized to the apparatus of a step-through inhibitory avoidance task for three sessions. On the fourth session, rats in the experimental group received an inescapable footshock (3 mA, 1 s) as they stepped from the lit side into the dark side of the task apparatus, whereas rats in the control group received the same amount of shock on a different apparatus. All rats were tested for retention in the task apparatus 1 day after shock training. The experimental rats showed better retention than the controls as they stayed longer in the lit side. Ensemble unit activities were recorded in the amygdala nuclei from the indwelling wire bundles during the acclimation and test sessions. The data collected from well-isolated amygdala units showed that neuronal discharge habituated from the first to the third acclimation session. In the test session, the experimental group, but not the control group, showed elevated firing rates in the CeA or BLC neurons located on either side of the brain. These findings provide the first piece of evidence showing that learning of an inhibitory avoidance task leads to an increase in amygdala neuronal discharges during a retention test.