Virulence determinants of Salmonella Gallinarum biovar Pullorum identified by PCR signature-tagged mutagenesis and the spiC mutant as a candidate live attenuated vaccine
Virulence determinants of Salmonella Gallinarum biovar Pullorum identified by PCR signature-tagged mutagenesis and the spiC mutant as a candidate live attenuated vaccine
复制标题
通过 PCR 特征标记诱变鉴定鸡沙门氏菌白痢生物变种的毒力决定因素以及作为候选减毒活疫苗的 spiC 突变体
DOI：
10.1016/j.vetmic.2013.11.024
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发表时间：
2014-01-31
影响因子：
3.3
通讯作者：
Chen, Xiang
中科院分区：
文献类型：
--
作者：
Geng, Shizhong;Jiao, Xinan;Chen, Xiang
鸡沙门氏菌鸡白痢（Salmonella gallinarum biovar Pullorum）鸡白痢（Gallinarum biovar Pullorum）是鸡白痢病（PD）的病原，给发展中国家的养禽业造成了巨大的经济损失。采用PCR-Signature Tagged Mutagenesis方法鉴定了S.鸡白痢生物变种和用于对抗该疾病的新型减毒活疫苗候选物。一个包含1800个签名标记的S.构建了鸡白痢生物变种突变体，并筛选了鸡的毒性相关基因。通过体内和体外竞争指数（CI）研究证实了10个突变体的减毒作用。转座子定位于SPI-1（2/10）、SPI-2（3/10）、毒力质粒（1/10）和非SPI基因（4/10）。一种高度减毒的spiC突变体在脾脏和肝脏中持续存在不到10天，并诱导了高水平的循环抗体和保护性免疫。spiC突变体是一种潜在的新疫苗候选者，用于鸡对抗这种疾病。(C)2013爱思唯尔有限公司版权所有。
Salmonella Gallinarum biovar Pullorum (S. Gallinarum biovar Pullorum) is the causative agent of pullorum disease (PD) in chickens which results in considerable economic losses to the poultry industries in developing countries. PCR-Signature Tagged Mutagenesis was used to identify virulence determinants of S. Gallinarum biovar Pullorum and novel attenuated live vaccine candidates for use against this disease. A library of 1800 signature-tagged S. Gallinarum biovar Pullorum mutants was constructed and screened for virulence-associated genes in chickens. The attenuation of 10 mutants was confirmed by in vivo and in vitro competitive index (CI) studies. The transposons were found to be located in SPI-1 (2/10 mutants), SPI-2 (3/10), the virulence plasmid (1/10) and non-SPI genes (4/10). One highly attenuated spiC mutant persisted in spleen and liver for less than 10 days and induced high levels of circulating antibody and protective immunity against oral challenge in young broiler chickens. The spiC mutant is a potential new vaccine candidate for use with chickens against this disease. (C) 2013 Elsevier B.V. All rights reserved.